Thioglycollate Medium w/ Hemin and Vitamin K is recommended for routine cultivation of fastidious anaerobic microorganisms. Also used for blood culturing and studying fermentation reactions. Certain anaerobes require addition of Vitamin K and hemin for their growth. Thioglycollate Medium with hemin and Vitamin K is recommended for use in the isolation and cultivation of fastidious or slow-growing obligatory anaerobic microorganisms present in clinical materials. It is also recommended for the isolation and cultivation of wide variety of aerobic and facultatively anaerobic microorganisms.
1. Gibbon R. J. and MacDonald J. B., 1960, J. Bacteriol., 80:164.2.Wilkins T. D., Chalgren S. L., Jimenez Ulat G., Drace C. R. , Johnson J. L., 1976, J. Clin Microbiol., 3:359.