Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for screening anaerobes in cosmetic products such as talcum powder.
Composition
| Ingredients | Gms/Litre |
|---|---|
| Tryptone | 15.000 |
| Soya peptone | 5.000 |
| Sodium chloride | 5.000 |
| Yeast extract | 5.000 |
| Hemin | 0.005 |
| Vitamin K1 | 0.010 |
| L-Cystine | 0.400 |
| Agar | 20.000 |
| Final pH (at 25°C) | 7.5±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 50.41 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates. It is recommended that the medium be reduced by keeping in anaerobic jar-incubator for 24 hours before use.
Principle And Interpretation
Anaerobic microorganisms have long been known as constituents of the normal bacterial flora of human and animal organisms. Both their pathogenic significance in medicine and their important role in food hygiene have, however, long been underestimated. During the past few years the importance of anaerobic microorganisms as pathogenic agents responsible for infectious diseases and the role they play in the microbial spoilage of food, cosmetics and water has been better appreciated. Extremely different spectra of anaerobic organisms are of importance for the examination of food, cosmetics and in the clinical microbiology (5). The present medium is a slight modification of Anaerobic Blood Agar formulated by Dowell et al which is a non-selective medium for the isolation and cultivation of a wide variety of obligately anaerobic microorganisms (1,2). Tryptone Soya Agar supplemented with additional agar, yeast extract, vitamin K1, hemin and cystine improves the growth of anaerobic organisms.
Tryptone, yeast extract and soya peptone in the medium provide carbon, nitrogenous compounds, and the vitamins and growth factors supply enrichment for growth of anaerobes. Sodium chloride helps in maintaining the osmotic equilibrium. Hemin, vitamin K1, cystine provide growth factors.
Type of specimen
Cosmetic products
Specimen Collection and Handling
Streak the specimen as soon as it is received in the laboratory. Minimize the exposure to air. Inoculate and incubate the plates under anaerobic conditions for minimum 48 hrs and up to 7 days. In order to determine the relationship to oxygen of each colony type present on Anaerobic Agar, inoculate and incubate the plates aerobically as well as anaerobically. Record the ability of organism to grow in absence of oxygen as either obligate anaerobe or non-anaerobe. Organisms failing to grow on the aerobic subculture plates may be presumed to be obligately anaerobic. After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 2.0% agar gel.
Colour and Clarity of prepared medium: Light amber coloured clear to slightly opalescent gel forms in Petri plates
Reaction: Reaction of 5.04% w/v aqueous solution at 25°C. pH: 7.5±0.2
pH: 7.30-7.70
Cultural Response
Cultural characteristics observed under anaerobic conditions after an incubation at 35-37°C for 48 hours
| Organism | Growth |
|---|---|
| Bacteroides fragilis ATCC 25285 | luxuriant |
| Bacteroides melaninogenicus ATCC 25611 | luxuriant |
| Peptostreptococcus anaerobius ATCC 27337 | luxuriant |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
Reference
- Dowell and Hawkins, 1979, CDC Laboratory manual, CDC, Atlanta
- Dowell, Lombard, Thompson and Armfield, 1977, CDC Laboratory manual, CDC, Atlanta
- Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
- Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.
- Ljungdahl L. G., Adams M. W., Barton L. L., Ferry J. G., Johnson M. K Biochemistry and Physiology of Anaerobic Bacteria. Microbiology.Springer publication
| Product Name | Anaerobic Tryptone Soya Agar |
|---|---|
| SKU | M975 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Ljungdahl L. G., Adams M. W., Barton L. L., Ferry J. G., Johnson M. K ., Biochemistry and Physiology of AnaerobicBacteria. Microbiology.Springer publication |
| Customized Product Available | No |
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