Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Yeast Extract Rose Bengal Broth Base
For the cold enrichment for the recovery of Yersinia enterocolitica and Yersinia pseudotuberculosis from food samples as per APHA.
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Composition
| Ingredients | Gms / Litre |
|---|---|
| Yeast extract | 5.000 |
| Disodium phosphate | 17.250 |
| Bile salts | 2.000 |
| Sodium chloride | 1.000 |
| Magnesium sulphate | 0.010 |
| Sodium pyruvate | 1.000 |
| Rose bengal | 0.040 |
| Final pH (at 25°C) | 7.9±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 26.3 grams in 900 ml distilled water. Heat if necessary, to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 20-25°C and aseptically add 100 ml of 4% filter sterilized sorbose solution. Mix well and dispense aseptically as desired.
Principle And Interpretation
Yersinioses are zoonotic infections that usually affect rodents, small animals and birds, while humans are accidental hosts.
Yersinia enterocolitica is a significant and invasive enteric pathogen that causes several well-recognized diseases, especially in younger persons, and several uncommon post-infection syndromes (1). Yersinia pseudotuberculosis causes a zoonotic disease with its natural reservoir being rodents, wild animals and game birds (2). Yeast Extract Rose Bengal Broth is formulated as recommended in APHA for enrichment of Yersinia species from foods (3), using the cold enrichment method. Yersinia species are psychrotropic and therefore grow at 4°C. Yeast extract provides essential nutrients. Bile salts inhibit gram-positive organisms. Various salts help in cold enrichment of Yersinia species.
Y. enterocolitica and Y. pseudotuberculosis can grow at 4°C, so primary enrichment is carried out in this medium for 9 days at 4°C or 3 days at 10°C (4). From this enrichment the organisms are further enriched in secondary selective enrichment medium such as PSTA Broth (M940) and then isolated by streaking onto selective plating media such as Yersinia Selective Agar (M843), SS Agar (M108) and MacConkey Agar (M082).
Quality Control
Appearance
Light yellow to pink homogeneous free flowing powder
Colour and Clarity of prepared medium
Reddish pink coloured clear solution without any precipitate.
Reaction
Reaction of the medium (2.63% w/v 90ml Base + 10 ml of 4% w/v sorbose) at 25°C. pH: 7.9±0.2
pH
7.70-8.10
Cultural Response
Cultural characteristics observed after incubation at 4°C for 9-10 days or at 10°C for 3 days with added sorbose solution.
Cultural Response
| Organism | Inoculum (CFU) | Recovery |
|---|---|---|
| Yersinia enterocolitica ATCC 27729 | 50-100 | good-luxuriant |
| Yersinia pseudotuberculosis ATCC 29833 | 50-100 | good-luxuriant |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
Reference
- Cover T. L. and Aber R. C., 1989, N. Engl. J. Med. 321:16-24
- Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology, 8th Ed., American Society for Microbiology, Washington, D.C.
- Speck M. L., (Ed.), 1984, Compendium of Methods for the Microbiological Examination of Foods, 2nd Ed., APHA, Washington, D.C.
- Schiemann D. A., 1982, Appl. Environ. Microbiol., 43:14.
| Product Name | Yeast Extract Rose Bengal Broth Base |
|---|---|
| SKU | M955 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Cover T. L. and Aber R. C., 1989, N. Engl. J. Med. 321:16-24 |
| Customized Product Available | No |
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