Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for transport and storage of Campylobacter species.
Composition
| Ingredients | g/L |
|---|---|
| Tryptone | 10.000 |
| Peptone | 10.000 |
| Dextrose (Glucose) | 1.000 |
| Yeast extract | 2.000 |
| Sodium chloride | 5.000 |
| Sodium bisulphite | 0.100 |
| Agar | 5.000 |
| Final pH (at 25°C) | 7.0±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 33.1 grams in 900 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add 100 ml sterile defibrinated sheep blood. Mix well and dispense aseptically in tubes as desired. Allow the tubes to cool in a upright position.
Principle And Interpretation
Despite improved techniques, isolation of Campylobacter species from foods may still be difficult because of delays in culturing specimens. Therefore methods that maintain the viability of Campylobacter during transport and storage of specimens are important, especially for specimens that are collected far away from the processing laboratories.
The primary objective of a transport medium is to maintain the clinical sample as near to their original state as possible with minimum deterioration and to minimize hazards to specimen handlers. This is achieved by using tightly fitting collection devises confined within proper protective containers (1). Wangs Medium is an enriched, semisolid medium as recommended by APHA (2) and described by Wang (3). It is used for the transport and storage of cultures of Campylobacter species from food such as undercooked meat, poultry, unpasteurised milk, bile and water (3). Wangs Semisolid Medium can be used for maintenance of Campylobacter species.
The medium contains tryptone and peptone which serve as source of nitrogen. Yeast extract provides additional nutritious growth factors. Dextrose is a source of carbon. Sodium bisulphite accelerates growth of organisms. Sodium chloride maintains the osmotic balance. Addition of 0.5% agar makes the medium semisolid for maintaining viability of cultures for transport and storage by providing a semisolid consistency to prevent oxygenation and spillage during transport (1). Addition of 5-10% defibrinated horse blood provides additional source of nutrition for growth.
Type of specimen
Isolated Microorganism from food
Specimen Collection and Handling
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (2). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- The sample should be transported immediately to the laboratory.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Semisolid, comparable with 0.5% Agar gel forms in tubes as butt.
Colour and Clarity of prepared medium: Medium amber coloured clear solution without any precipitate.
Reaction: Reaction of 3.31% w/v aqueous solution at 25°C. pH : 7.0±0.2
pH: 6.80-7.20
Cultural response: Cultural characteristics observed after an incubation at 35-37°C for 5 days with added sterile defibrinated sheep blood.
Growth: Growth observed upon subculturing on Campyloblacter Agar Base (M994).
| Organism | Growth |
|---|---|
| Campylobacter jejuni | luxuriant |
| subsp jejuni 29428 (00156*) |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored, dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with samples must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | Wang’s Semisolid Medium |
|---|---|
| SKU | M918 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Koneman E. W., Allen S. D., Janda W. M., Schreckenberger P. C., Winn W. C. Jr., 1992, Colour Atlas and Textbook ofDiagnostic Microbiology, 4th Ed., J. B. Lippinccott Company |
| Customized Product Available | No |
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