Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition
| Ingredients | Gms/Litre |
|---|---|
| Casein enzymic hydrolysate | 17.000 |
| Papaic digest of soyabean meal | 3.000 |
| Sodium chloride | 5.000 |
| Dipotassium hydrogen phosphate | 2.500 |
| Saccharose | 5.000 |
| Bromo thymol blue | 0.025 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 32.5 grams in 1000 ml. distilled water. Heat if necessary to dissolv the medium completely. Dispense and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Saccharose Broth is used in studying fermentation reaction in colon bacteria (1). Casein enzymic hydrolysate and papaic digest of soyabean meal provide essential nutrients for bacterial metabolism. Saccharose provides the fermentable carbohydrate source for the bacteria. Bromothymol blue is a pH indicator. Sodium chloride maintains osmotic equilibrium.
Quality Control
Appearance: Cream to Yellow coloured homogeneous free flowing powder
Colour and Clarity of prepared medium: Bluish green coloured clear to very slightly opalescent solution.
Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
Cultural Response
| Organism | Inoculum (CFU) | Growth | Acid | Gas |
|---|---|---|---|---|
| Citrobacter freundii ATCC 8090 | 50-100 | luxuriant | positive reaction, yellow colour | positive reaction |
| Escherichia coli ATCC 25922 | 50-100 | luxuriant | negative reaction, no colour change | negative reaction |
| Klebsiella pneumoniae ATCC 13883 | 50-100 | luxuriant | positive reaction, yellow colour | positive reaction |
| Proteus vulgaris ATCC 13315 | 50-100 | luxuriant | positive reaction, yellow colour | positive reaction |
| Salmonella Typhimurium ATCC 14028 | 50-100 | luxuriant | negative reaction, no colour change | negative reaction |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Saccharose Broth |
|---|---|
| SKU | M844 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Oskar Klotz, Temporary Alteration of character of an organism belonging to the colon group. Journal of Medical Research,1994,6,P-475 |
| Customized Product Available | No |
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