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Feeley Gorman Agar (F.G. Agar)

Name: Feeley Gorman Agar (F.G. Agar)
Brand: HiMedia Laboratories
SKU: M811
Availability: InStock

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M811

Used for isolation and presumptive identification of Legionella species.

Description

Intended Use

Used for isolation and presumptive identification of Legionella species.

Composition

Ingredients	g/L
AcicaseTM#	17.500
HM peptone B ##	3.000
Starch	1.500
L-Cysteine hydrochloride	0.400
Ferric pyrophosphate, soluble	0.250
Agar	17.000
Final pH (at 25°C)	6.9±0.2

**Formula adjusted, standardized to suit performance parameters
#Equivalent to casein acid hydrolysate
# #Equivalent to Beef extract

Directions

Suspend 39.65 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C.Mix well and pour into sterile Petri plates.

Principle And Interpretation

Feeley et al formulated (1,2) Feeley Gorman Agar, which is used as nonselective enrichment medium for isolation of Legionella species. Legionella is a gram-negative bacterium, including species that cause legionellosis or Legionnaires' disease, most notably L. pneumophilia (3). Legionella species are the causative agent of the human Legionnaires' disease and the lesser form, Pontiac fever. Legionella transmission occurs via aerosols- inhalation of mist droplets containing the bacteria. Person-to-person transmission of Legionella has not been demonstrated (4).
Legionella are nutritionally fastidious and require L-cysteine and iron salts for their growth, which are provided in the medium. Legionella species are highly pathogenic microorganisms. Certain safety precautions must be taken when handling Legionella cultures.
Acicase™, HM peptone B, L-cysteine hydrochloride and ferric pyrophosphate act as sources of nutrients. It is recommended to inoculate F.G. Agar and Legionella Agar (M809) with supplements simultaneously, as Legionella usually do not grow initially on F.G. agar. Legionella species can be identified by their characteristic fluorescence in presence of UV light (5,6). Since Legionella disease is primarily a pulmonary infection, prevention and containment of aerosols is essential (7).

Type of specimen

Clinical samples - Urine, etc.; Water samples

Specimen Collection and Handling

For clinical samples follow appropriate techniques for handling specimens as per established guidelines (8,9).
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (10).
After use, contaminated materials must be sterilized by autoclaving before discarding. After handling with Legionella species sample decontaminate work surface with either 5% hypochlorite or 5% phenil (carbolic acid).

Warning and Precautions

In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.

Limitations

Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
Incubation should be carried out in the presence of 2.5% carbon dioxide but if it exceeds the limit, Legionella growth is inhibited due to formation of acidic condition.
Further biochemical and serological tests must be performed for confirmation.

Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

Quality Control

Appearance
Cream to yellow homogeneous free flowing powder

Gelling
Firm, comparable with 1.7% agar gel.

Colour and Clarity of prepared medium
Yellow coloured, clear to slightly opalescent gel forms in Petri plates

Reaction
Reaction of 4.0% w/v aqueous solution at 25°C. pH: 6.9±0.2

pH
6.70-7.10

Cultural Response

Cultural characteristics observed in presence of 2.5% Carbon dioxide (CO2) after an incubation at 35-37°C for 4 days.

Organism	Growth	Fluorescence under 366 nm
Legionella bozemanni ATCC 33217	good-luxuriant	blue-white
Legionella micdadei ATCC 33218	good-luxuriant	none
Legionella pneumophila ATCC 33153	good-luxuriant	bright yellow

Storage and Shelf Life

Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.

Disposal

User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (8,9).

Product Information

More Information
Product Name	Feeley Gorman Agar (F.G. Agar)
SKU	M811
Product Type	Regular
Physical Form	Powder
Origin	Animal
Packaging type	HDPE
References	1. Feeley J. C. et al, 1978, J. Clin. Microbiol., 8(3): 320.2.Feeley J. C. et al, 1979, J. Clin. Microbiol., 10(4):437.3.Ryan K. J., Ray C. G. (Eds.), 2004, Sherris Medical Microbiology, 4th Edition, McGraw Hill.4.Winn, W. C. Jr. ,1996, Legionella (In: Baron's Medical Microbiology, Barron, S. et al, (Eds.), 4th Edition, University ofTexas Medical Branch5.Herbert G. A. et al, 1959, Ann. Intern, Med., 92(1):45.6.Herbert G. A. et al, 1980, Ann. Intern. Med., 92(1):53.7.MacFaddin J. F., Vol. I, 1985, Media for Isolation Cultivation-Identification-Maintenance of Medical Bacteria, Williamsand Wilkins, Baltimore/ London, pg.307-308.
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