Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for microbiological assay of Bleomycin using Mycobacterium smegmatis.
Composition
| Ingredients | g/L |
|---|---|
| Peptone | 10.000 |
| HM peptone B # | 10.000 |
| Sodium chloride | 3.000 |
| Agar | 17.000 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract
Directions
Suspend 40 grams in 1000 ml purified / distilled water containing 10 ml glycerol. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Coolt to 45-50°C. Mix well and pour into sterile Petri plates.
Advice: Recommended for the microbiological assay of Bleomycin
Principle And Interpretation
This medium is formulated in accordance to CFR (1). This medium is employed widely as base agar for agar diffusion assay of Bleomycin using Mycobacterium smegmatis. The nutrients essential for growth of test organism is provided by Peptone and HM peptone B in this medium. Agar provides excellent solid substratum for support and overlaying of seed agar, for the assay of Bleomycin. Addition of glycerol is important for provision of carbon to the test organism.
To perform the antibiotic assay the Base Agar should be prepared on the same day as the test. For the cylinder method, a base layer of 10 ml is required. Once the base medium has solidified, seed layer inoculated with the standardized culture can be overlaid. Even distribution of the layer is important.
Type of specimen
Pharmaceutical sample
Specimen Collection and Handling
For pharmaceutical sample samples follow appropriate techniques for handling specimens as per established guidelines (1). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Freshly prepared medium plates must be used or it may result in erroneous results.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.7% agar gel.
Colour and Clarity of prepared medium
Medium amber coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 4.0% w/v aqueous solution containing 1% glycerol at 25°C. pH: 7.0±0.2
pH
6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at 36-37.5 for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Antibiotics assayed |
|---|---|---|---|---|
| Mycobacterium smegmatis ATCC 607 | 50-100 | luxuriant | >=50% | Bleomycin |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and use freshly prepared medium. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | Antibiotic Assay Medium No. 35 |
|---|---|
| SKU | M798 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Tests and Methods of Assay of Antibiotics and Antibiotic containing Drugs, FDA, CFR, 1983Title 21, Part 436, Subpart D,Washington, D.C.: U.S. Government Printing Office, paragraphs 436, 100-436, 106, p. 242-259, (April 1). |
| Customized Product Available | No |
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