Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use:
Recommended for for isolation of fastidious microorganisms from clinical specimens under reduced oxygen tension.
Composition
| g/L | |
|---|---|
| Proteose peptone | 10.000 |
| Tryptose | 10.000 |
| HM peptone B# | 3.000 |
| Dextrose (Glucose) | 0.500 |
| Corn starch | 1.000 |
| Sodium chloride | 5.000 |
| Nicotinamide | 0.050 |
| p-Amino benzoic acid (PABA) | 0.050 |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
# - Equivalent to Beef extract
Directions
Suspend 29.6 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add 0.15% v/v sterile waterlysed blood (water:blood :: 3:1) of 5% sterile blood. Alternatively add 5% partially lysed blood. Mix well and dispense as desired.
Principle And Interpretation
Fastidious microorganisms such as Haemophilus and Neisseria require the addition of X and V- growth factors for in vitro cultivation (1). Casman (1, 2, 3) described a blood-enriched medium for cultivation of Haemophilus and Gonococci (1). The medium was developed to replace the previously described formulations that required time-consuming preparations using fresh and heated blood and meat infusion to supply the essential nutrients for growth of these fastidious organisms (2, 3). Blood supplies factor-X (hemin) and factor-V (Nicotinamide Adenine Dinucleotide), which is required for growth of Haemophilus influenzae. Sheep blood lacks factor-V due to NADase, an enzyme that destroys factor- V (4). Horse and rabbit blood supplies both the factor X and factor V, and are relatively free of NADase activity, therefore it is preferred over sheep blood. Nicotinamide is added to medium to inhibit nucleotidase of erythrocytes that may destroy factor V.
Proteose peptone, tryptose and HM peptoneB provide amino acids and other complex nitrogenous nutrients. Dextrose improves growth of pathogenic cocci. Corn starch prevents fatty acids from inhibiting the growth of Neisseria gonorrhoeae, without interfering with haemolytic reaction. Corn starch also neutralizes the inhibitory action of dextrose. Inoculate the medium as soon as the specimen arrives at the laboratory.
Type of specimen
Clinical samples - vaginal swabs, rectal swabs, etc.
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (5,6). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic Use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further biochemical and serological tests must be carried out for further identification.
Performance And Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Basal medium: Yellow coloured clear to slightly opalescent solution. After addition of blood: Cherry red coloured opalescent solution in tubes
Reaction
Reaction of 2.96% w/v aqueous solution at 25°C. pH: 7.2±0.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed with added water-lysed blood, after an incubation at 35-37°C for 40-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Haemophilus influenzae ATCC 35056 | 50-100 | good-luxuriant |
| Neisseria meningitidis ATCC 13090 | 50-100 | luxuriant |
| Streptococcus mitis ATCC 9895 | 50-100 | luxuriant |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Casman Broth Base |
|---|---|
| SKU | M766 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Casman, 1947, Am. J. Clin. Pathol., 17:281. 2.Casman, 1942, J. Bact., 43:33. 3.Casman, 1947, J. Bact., 53:561. 4.Krunveide and Kuttner, 1938, J. Exp. Med., 67:429. 5.Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition 6.Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015), Manualof Clinical Microbiology, 11th Edition. Vol. 1 |
| Customized Product Available | No |
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