Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition**
| Ingredients | Gms / Litre |
|---|---|
| Casein enzymic hydrolysate | 17.000 |
| Papaic digest of soyabean meal | 3.000 |
| Dextrose | 6.000 |
| Sodium chloride | 2.500 |
| Sodium thioglycollate | 0.500 |
| L-Cystine | 0.250 |
| Sodium sulphite | 0.100 |
| Calcium carbonate | 0.100 |
| Agar | 0.700 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 30.15 grams in 1000 ml distilled water. Heat just to boiling. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 25°C and store in a cool, dry place preferably below 25°C.
Note: Due to the presence of calcium carbonate, the prepared medium forms opalescent solution with white precipitate.
Principle And Interpretation
Thioglycollate Medium with CaCO3 is a modification of the original medium of thioglycollate described by Brewer (1) and is recommended for maintenance of stock cultures.
Casein enzymic hydrolysate, soyabean meal and L-cystine provides amino acids and other nitrogenous substances to support bacterial growth. Yeast extract provides the B-complex vitamins. Sodium chloride provides essential ions. Dextrose is an energy source. The incorporation of calcium carbonate helps to neutralize acid produced during growth (2, 3). The reducing action provided by sodium thioglycollate and sodium sulphite binds molecular oxygen, thereby maintaining a low Eh. (4). A small amount of agar is added to retard the absorption of oxygen by reducing convection currents in the medium.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light yellow coloured very slightly opalescent solution over a slight white precipitate on standing.
Reaction
Reaction of 3.01% w/v aqueous solution at 25°C. pH: 7.0±0.2
pH
6.80-7.20
Cultural Response
M765: Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Clostridium perfringens ATCC 12924 | 50-100 | luxuriant |
| Clostridium sporogenes ATCC 11437 | 50-100 | luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Thioglycollate Medium w/ CaCO3 |
|---|---|
| SKU | M765 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Brewer J.H 1940. J Bacteriol 39:10. 2.Vera H.D., 194 4.J. Bacteriol 47:59-70. 3.Reischelderfer C, and J.I. Mangels. 199 2.Cultural media for anerobes p2. 3.1-2. 3. 8.In H.D Isenberg (ed.), ClinicalMicrobiology procedures handbook, vol. 1-American Society for Microbiology, Washington. 4.MacFaddin., J.F 198 5.Media for isolation-cultivation-identification-maintenance of medical bacteria, vol.1 William andWilkins, Baltimore. |
| Customized Product Available | No |
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