Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
With added blood and tellurite it is used for isolation of Corynebacterium diphtheriae from clinical and non-clinical samples.
Composition
| Ingredients | g/L |
|---|---|
| Proteose peptone | 20.000 |
| Dextrose (Glucose) | 2.000 |
| Sodium chloride | 5.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.4±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 42.0 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Aseptically add sterile 5% v/v defibrinated blood and sterile PTe 1% Selective Supplement (1 ml per vial)(FD052). Mix well before pouring into sterile Petri plates.
Principle And Interpretation
Corynebacterium diphtheriae is the causative agent of diphtheria, an acute communicable disease manifested by both local infection of the upper respiratory tract and the systemic effects of a toxin, which are most notable in the heart and peripheral nerves.C. diphtheriae is most often isolated from the nasopharynx or skin lesions of patients with diphtheria (1). Dextrose Proteose Peptone Agar is used for the isolation of C.diphtheriae, in combination with blood and tellurite. A selective serum medium containing tellurite was described by Conradi and Troch for isolating C.diphtheriae (2). This medium later on underwent modification by different authors in which they used heated Blood Agar Tellurite or Blood Agar Tellurite Arsenate Medium (3,4,5). McGuigan and Frobisher had used a Cystine Tellurite Blood Agar for C.diphtheriae (6). Without the inclusion of blood and tellurite, this medium is recommended as a general laboratory medium. With added tellurite and blood, this medium permits the isolation of C.diphtheriae.
Proteose peptone serves as source of carbon, nitrogen, vitamins and minerals. Dextrose serves as an energy source. Sodium chloride helps to maintain the osmotic equilibrium. Potassium tellurite serves as a selective agent.
Type of specimen
Clinical samples- sputum samples, throat swab, etc
Specimen Collection and Handling:
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (7,8).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions:
In Vitro diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets. Use only. Read the label before opening the container. Wear protective gloves
Limitations :
- Specimens if enriched on Loeffler medium, can give better growth of Corynebacterium species.
- Other organisms such as Staphylococci, Streptococci will grow as minute black colonies due to tellurite reduction, hence Corynebacterium should be confirmed by gram staining and other biochemical test.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature..
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel
Colour and clarity of Medium
Reddish brown coloured, opaque gel forms in Petri plates
Reaction
Reaction of 4.2% w/v aqueous solution at 25°C. pH: 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed with added 5%v/v sterile defibrinated blood and PTe 1% Selective Supplement (1 ml per vial) (FD052), after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of Colony |
|---|---|---|---|---|
| Corynebacterium diphtheriae ATCC 11913 | 50-100 | good-luxuriant | >=50% | black |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | Dextrose Proteose Peptone Agar Base |
|---|---|
| SKU | M734 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Murray P. R., Baron E. J, Pfaller M. A, Tenover F. C, Yolken R .H. ( Eds.) 1995, Manual of Clinical Microbiology, 6thEd. ASM Press, Washington D.C. 2.Conradi and Troch, 1912, Muench. Wochschr., 59:1652. 3.Anderson J. S., Happold F. C, McLeod J. W. and Thompson J. G., 1931, J. Path. Bacteriol., 34:667. 4.Horgan E. S. and Marshall A., 1932, J. Hyg., 32:544. 5.Wilson S., 1934, J. Path. Bact., 38:114. 6.McGuigan and Frobisher, 1936, J. Infect. Dis., 59:22 |
| Customized Product Available | No |
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