Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition**
| Ingredients | Gms / Litre |
|---|---|
| Sodium thiosulphate | 5.000 |
| Dipotassium hydrogen phosphate | 0.100 |
| Sodium bicarbonate | 0.200 |
| Ammonium chloride | 0.100 |
| Agar | 20.000 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 25.4 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Add excess of calcium carbonate if desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.
Principle And Interpretation
The various transformations of sulphur in the biosphere can be summed up as a cyclic reaction involving
- a) decomposition of organic sulphur compounds into subunits which are in turn converted into inorganic compounds through a process of mineralization,
- b) assimilation of sulphur into the protoplasm of microorganisms, a process referred to as immobilization,
- c) oxidation of inorganic sulphur compounds into elemental sulphur, and
- d) reduction of sulphate.
Desulfovibrio desulfuricans is a species of sulphate-reducing bacteria that reduce inorganic sulphate into hydrogen sulphide. Thiosulphate Agar is used for cultivation of sulphur metabolizing bacteria (1). D. desulfuricans reduces sodium thiosulphate in the medium to hydrogen sulphide (1). Other salts serve to fulfill the essential ion requirement of bacteria.
Quality Control
Appearance
Cream to yellow coarse powder
Gelling
Firm, comparable with 2.0% Agar gel.
Colour and Clarity of prepared medium
Light yellow coloured clear to slightly opalescent gel forms in Petri plates or tubes.
Cultural Response
M726: Cultural characteristics observed after an incubation at 30°C for 7 days under anaerobic conditions.
| Organism | Growth |
|---|---|
| Desulfovibrio desulfuricans ATCC 13541 | luxuriant |
Storage and Shelf Life
Store below 30°C in tightly closed container and use freshly prepared medium. Use before expiry date on the label.
| Product Name | Thiosulphate Agar |
|---|---|
| SKU | M726 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Chemically defined |
| Packaging type | HDPE |
| References | 1. Subba Rao N. S., Soil Microorganisms and Plant Growth, (Oxford and IBH Publishing Co.) |
| Customized Product Available | No |
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Sulphate API Agar w/o Sodium Lactate
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