Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for germinating seeds of leguminous plants while studying the nodulating ability of Rhizobium isolates.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Calcium phosphate | 1.000 |
| Dipotassium hydrogen phosphate | 0.200 |
| Magnesium sulphate | 0.200 |
| Sodium chloride | 0.200 |
| Ferric chloride | 0.100 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 16.7 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and dispense as desired.
Principle And Interpretation
Rhizobium is a soil bacterium that has great environmental and agricultural importance because of their symbiotic association with leguminous plants. They are responsible for most of the atmospheric nitrogen fixed on the earth (1). Rhizobium is a free-living bacterium, which grow well on a nitrogen free medium. These bacteria utilize atmospheric nitrogen gas for their cell protein synthesis. This cell protein is then mineralised in soil after the death of the cells thereby contributing towards the nitrogen availability to the crop plants (5). Jensen Seedling Agar, a nitrogen free medium, is used for germinating seeds of leguminous plants while studying the nodulating ability of Rhizobium species (3). Calcium stimulates nodulation when present as chloride or sulphate. Sodium chloride maintains the osmotic balance of the medium. Dipotassium phosphates provide buffering to the medium. Magnesium sulphate and ferric chloride are sources of ions that simulate metabolism.
Type of specimen
Soil sample- root system of the leguminous plant
Specimen Collection and Handling
For soil samples follow appropriate techniques for handling specimens as per established guidelines (5). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to beige homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light cream coloured, clear to slightly opalescent gel with a slight precipitate.
Reaction
Reaction of 1.67% w/v aqueous solution at 25°C. pH: 7.0±0.2
pH 6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at 30°C for 7 days.
| Organism | Growth |
|---|---|
| Rhizobium japonicum ATCC 10324 | luxuriant |
| Rhizobium leguminosarum ATCC 10004 | luxuriant |
| Rhizobium meliloti ATCC 9930 | luxuriant |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,4).
| Product Name | Jensen Seedling Agar |
|---|---|
| SKU | M718 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Chemically defined |
| Packaging type | HDPE |
| References | 1.Clemence Chaintrevil, Eric Giraud, Yves Prin et al, Appl. Environ. Microbiol., 2000, December; 66 (12): 5437 - 5447. 2.Subba Rao N. S., 1977, In: Soil Microorganisms and Plant Growth, Oxford and IBH Publishing Co., New Delhi, Pages254-255. 3.Jensen H. L., Nitrogen fixation in leguminous plants. I., Proc. Int. Soc. NSW, 1942; 66:68 - 108. |
| Customized Product Available | No |
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