Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for isolation of Beijerinckia.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Potassium dihydrogen phosphate | 0.800 |
| Sucrose | 20.000 |
| Dipotassium hydrogen phosphate | 0.200 |
| Magnesium sulphate | 0.500 |
| Ferric chloride | 0.100 |
| Sodium molybdate | 0.005 |
| Agar | 15.000 |
| Final pH (at 25°C) | 6.5±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 36.6 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Beijerinckia characterizes its members as non-symbiotic, aerobic chemoheterotrophic bacteria with the ability to fix atmospheric nitrogen (2). The free living bacteria having the ability to fix molecular nitrogen can be distinguished into obligate aerobic facultative aerobic and anaerobic organisms. Beijerinckia is an obligate aerobic bacterium. Beijerinckia Medium is used for the isolation of Beijerinckia species (1). Though members of this species utilize a wide range of multicarbon compounds, sugars are the preferred growth substrates. Sucrose is the energy source, phosphates serve as buffers and magnesium sulphate, ferric chloride and sodium molybdate act as trace elements.
Type of specimen
Soil samples.
Specimen Collection and Handling
For soil samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (5). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- 1. Some species may show poor growth due to nutritional variations.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
White to cream homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Colourless clear to slightly opalescent gel forms in Petri plates.
Reaction
Reaction of 3.66% w/v aqueous solution at 25°C. pH : 6.5±0.2
pH
6.30-6.70
Cultural Response
Cultural characteristics observed after an incubation at 20-30°C for 18-48 hours.
| Organism | Growth |
|---|---|
| Beijerinckia indica ATCC 21423 | luxuriant |
| Beijerinckia mobilus | luxuriant |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Beijerinckia Medium |
|---|---|
| SKU | M708 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Chemically defined |
| Packaging type | HDPE |
| References | 1. Duorkir M. et al (Ed.), 1999, 3rd Ed. N.Y. 2.Becking J.H, 1959, PC soil, 11 193 - 206. |
| Customized Product Available | No |
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