Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Potato Carrot Agar is used for the reproduction of Pyronema domesticum.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Carrot, infusion from | 200.000 |
| Potatoes, infusion from | 250.000 |
| Agar | 15.000 |
Final pH (at 25°C): 6.5±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 24 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Potato Carrot Agar is used for the reproduction of Pyronema domesticum and for the cultivation and maintenance of Actinoplanes awajinensis, Actinoplanes nirasakiensis, Amorphosphorangium auranticolor, Streptomyces flavus and Thermoactinomyces vulgaris.
The medium is prepared based on formula originally designed by Langeron and Vanbreuseghem in 1952 (1) and recommended by Onions (2) and Atlas and Parks (3). This is a weak or starvation medium suitable for conservation of organisms. The medium restricts mycelial growth and promotes relatively high proportion of spores. The fungi grown on this medium when subsequently inoculated into richer medium yield rich growth (2).
Carrot and potato infusions provide the necessary carbohydrates, proteins, minerals and vitamins for limited growth of organisms, thereby providing an environment only for the existence rather than their growth.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Yellow coloured clear to slightly opalescent gel forms in Petri plates
Reaction: Reaction of 2.4% w/v aqueous solution at 25°C. pH : 6.5±0.2
pH: 6.30-6.70
Cultural Response: Cultural characteristics observed after an incubation at 20-25°C for 48-72 hours.
| Organism | Growth |
|---|---|
| *Aspergillus brasiliensis ATCC 16404 | good-luxuriant |
| Pyronema domesticum | good-luxuriant |
| Saccharomyces cerevisiae ATCC 9763 | good-luxuriant |
| Saccharomyces uvarum ATCC 9080 | good-luxuriant |
Storage and Shelf Life
Store below 30°C in tightly closed container and prepared media at 2-8°C. Use before expiry date on label.
Reference
- Langeron M. and Vanbreuseghem R., 1952, In “Precis de Mycologie”, p.408, Masson et Cie, Paris.
- Onions A. H. S., 1971, In “Methods in Microbiology”, Edited by Booth C., The Series edited by Norris J. R. and Ribbons D. W., Academic Press, London.
- Atlas R. M. and Parks L. C., (Ed.), 1993, Handbook of Microbiological Media CRC Press, Boca Raton / London.
| Product Name | Potato Carrot Agar |
|---|---|
| SKU | M696 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Langeron M. and Vanbreuseghem R., 1952, In “Precis de Mycologie”, p.408, Masson et Cie, Paris. 2.Onions A. H. S., 1971, In “Methods in Microbiology”, Edited by Booth C., The Series edited by Norris J. R. and RibbonsD. W., Academic Press, London. 3.Atlas R. M. and Parks L. C., (Ed.), 1993, Handbook of Microbiological Media CRC Press, Boca Raton / London. |
| Customized Product Available | No |
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