This medium is employed widely as
base agar for agar diffusion assay of Bleomycin using Mycobacterium smegmatis . It is also used for preparing the inoculum of Mycobacterim smegmatis for assay. To perform the antibiotic assay the Base Agar should be prepared on the same day as the test. For the cylinder method, a base
layer of 21 ml is required. Once the base medium has solidified, seed layer inoculated with the standardized culture can be overlaid.
1. British Pharmacopoeia, 2011, The Staionery Office , British Pharmacopoeia 2.European Pharmacopoeia, 2011, European Department, for the Quality of Medicines.