Culture Medium for RWC (Disinfectant Test Broth) (RWC Medium) is recommended for determination of phenol coefficients of disinfectants using Salmonella typhi as a test organism. Salmonella Typhi grows luxuriantlly on the media.The medium contains ingredients like HM peptone B and peptone, which provide necessary nutrients to the growth of Salmonella Typhi when used as test organism. Presence of sodium chloride balances the osmotic equilibrium.