Minimal Broth, Davis w/o Dextrose

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M390
For the isolation and characterization of nutritional mutants of Escherichia coli.


Minimal Broth Davis w/o Dextrose is recommended for the isolation and characterization of nutritional mutants of Escherichia coli. A cell suspension of wild type Escherichia coli is irradiated and cultured on Minimal Agar and incubated at 35°C for 24hours. The isolated colonies are cultured in tubes of Minimal Broth Davis (M389) and Minimal Broth Davis without Dextrose(M390). After 24 hours incubation at 35°C growth in the Minimal Broth, Davis and absence of growth in the Minimal BrothDavis without Dextrose indicates a mutant.
More Information
Product Name Minimal Broth, Davis w/o Dextrose
SKU M390
Product Type Regular
Physical Form Powder
Origin Chemically defined
Packaging type HDPE
References 1. Davis B. D., 1949, Proc. Natl Acad. Sci, 35:1.
2.Lederberg J., 1950, Methods in Med. Res., 3:5.
3.Nester E. W., Schafer M. and Lederberg J., 1963, Genetics, 48:529.
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