B12 Inoculum Broth

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M206
Used for preparing the inoculum of Lactobacillus leichmannii ATCC 7830 in the microbiological assay of Vitamin B12.


Used for preparing the inoculum of Lactobacillus leichmannii ATCC 7830 in the microbiological assay of Vitamin B12. Recommended by USP. For preparing inoculum, the culture is grown in 5 ml sterile B12 Inoculum Broth for 18 to 24 hours at 35°C and then the culture is centrifuged to obtain cell sediment. The supernatant is decanted and the cells are suspended in B12 Assay Medium (M036).This cell suspension is used as an inoculum after adjusting its density.
More Information
Product Name B12 Inoculum Broth
SKU M206
Product Type Regular
Physical Form Powder
Origin Animal
Packaging type HDPE
References 1.Weber and Black, 1948, Soap and Sanitary Chemicals, 24:134.
2.Weber and Black, 1948, Am. J. Public Health, 38:1405.
3.Favero (chm.), 1967, Microbiological Sampling of Surfaces, Biological Contamination Control Committee, American Asso. for Contamination Control.
4.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition.
5.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1.
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