Tryptose Serum Broth Base (Modified Newings Tryptose Broth Base) is recommended for for the cultivation of Mycoplasma species. The medium ingredients and all the supplements should be free of any toxic substances even in small amounts.Tryptose serves as a source of nitrogeneous and carbonaceous compounds, long chain amino acids, vitamins and other growth nutrients. Sodium chloride maintains osmotic balance. Dextrose serves as an energy source. Yeast extract provides vitamins especially Group B Vitamins. Glycerol serves as a carbon sorce. Penicillin G and thallium acetate inhibits contaminating flora. Pig serum provides good growth.