TSB Cap4 w/Tween 80 (Twin Pack)

Intended Use

Recommended for determining efficiency of sanitization of containers, equipment surfaces, water miscible cosmetics etc.

Composition**

Final pH (at 25°C): 7.3±0.2

**Formula adjusted, standardized to suit performance parameters

Directions

Suspend 37.5 grams of Part A in 800 ml purified / distilled water. Separately add 42.5 ml of Part B in 100 ml purified/ distilled water. Mix well and add to Part A solution. Make up the volume to 1000 ml. Heat if necessary to dissolve the medium completely. Mix well and dispense in tubes or flasks or as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Note : Medium may show haziness after sterilization but on cooling the medium becomes clear.

Principle And Interpretation

Tryptone Soya lecithin Broth with Tween 80 & Tamol is used for the detection and enumeration of microorganisms for products of sanitary importance, water miscible cosmetics and containing antimicrobials or preservatives (1).

Tryptone, soya peptone and peptone provides carbonaceous and nitrogenous compounds, long chain amino acids, vitamins and other essential growth nutrients for the microorganisms. Dextrose (Glucose) is the source of carbohydrate. Sodium chloride maintains the osmotic balance. Dipotassium hydrogen phosphate buffers the medium. Soya lecithin, polysorbate 80 (Tween 80) and tamol act as neutralizing agents that neutralizes the activity of antimicrobial agents. Lecithin and polysorbate 80 neutralizes quaternary ammonium compounds, parahydroxy benzoates and substituted phenolics.

Collection of samples from areas before and after the treatment with disinfectant evaluates cleaning procedures in environmental sanitation (4).

Type of specimen

Environmental samples

Specimen Collection and Handling

For Environmental samples follow appropriate techniques for handling specimens as per established guidelines (1). After use, contaminated materials must be sterilized by autoclaving before discarding.

Warning and Precautions

Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.

Limitations

1. This medium is general purpose medium and may not support the growth of fastidious organisms.

Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

Quality Control

Appearance Part A: Cream to yellow homogeneous free flowing powder. Part B: Yellow to amber viscous solution.

Colour and Clarity of prepared medium Light to medium amber coloured, clear to slightly opalescent solution

Reaction Reaction of 3.75% w/v aqueous solution of Part A and 4.2 ml of Part B at 25°C. pH : 7.3±0.2

pH 7.10-7.50

Cultural response Cultural characteristics observed after an incubation at-specified temprature and period.

Sterility Testing- Growth promotion+Validation

Key : (*) - Corresponding WDCM numbers. (#) - Formely known as Aspergillus niger

Storage and Shelf Life

Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.

Disposal

User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).

Reference

1. Hall and Hartnett, 1964, Public Health. Rep., 79:1021.
2. Isenberg, H.D. Clinical Microbiology Procedures Handbook 2^nd Edition.
3. Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.
4. Murray PR, Baron, Pfaller, and Yolken (Eds.), 2003, In Manual of Clinical Microbiology, 8th ed., ASM, Washington, D.C.