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Soyabean Casein Digest Medium w/ Mannitol, Sterile Powder
For the evaluation of sterility in manufacturing process. It can also be used For cultivation of a wide variety of microorganisms.
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Gamma irradiated sterile powder recommended for the evaluation of sterility in manufacturing process. It can also be used for cultivation of a wide variety of microorganisms.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Pancreatic digest of casein | 17.000 |
| Papaic digest of soyabean meal | 3.000 |
| Sodium chloride | 5.000 |
| Phenol red | 0.018 |
| Mannitol | 5.000 |
Final pH ( at 25°C): 7.3±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Sterile powder can be used directly for the evaluation of sterility in manufacturing process. For sterile liquid medium aseptically add 30.02 grams in 1000 ml sterile distilled / purified water. Heat if necessary to dissolve the medium completely. DO NOT AUTOCLAVE OR OVERHEAT. Excessive heating is detrimental. Dispense aseptically in sterile tubes or flasks as desired.
Principle And Interpretation
Soyabean Casein Digest Medium is recommended as a sterility testing medium (1,2). Soyabean Casein Digest Medium w/ Mannitol is modified by addition of mannitol as the fermentable carbohydrate and phenol red as the indicator. It is also used for the sensitivity testing by the tube dilution method for antimicrobial agents (3).
The combination of pancreatic digest of casein and papaic digest of soyabean meal makes this medium nutritious by providing amino acids and long chain peptides for the growth of microorganisms. Sodium chloride maintains the osmotic balance of the medium. Mannitol is the fermentable carbohydrate and phenol red is the pH indicator. Organisms ferment mannitol and produce acid which is indicated by the colour change of the medium from red to yellow.
Quality Control
Appearance: Light yellow to pink homogeneous free flowing powder
Colour and Clarity of prepared medium: Red coloured clear solution without any precipitate.
Reaction: pH of 3.0% w/v aqueous solution at 25°C. pH : 7.3±0.2
pH: 7.10-7.50
Cultural Response
Cultural characteristics observed after an incubation at 30-35°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Colour change |
|---|---|---|---|
| Staphylococcus aureus ATCC 6538 | 50-100 | luxuriant | Red to yellow |
| Staphylococcus aureus ATCC 25923 | 50-100 | luxuriant | Red to yellow |
| Escherichia coli ATCC 8739 | 50-100 | luxuriant | Red to yellow |
| Escherichia coli ATCC 25922 | 50-100 | luxuriant | Red to yellow |
| Escherichia coli NCTC 9002 | 50-100 | luxuriant | Red to yellow |
| Bacillus subtilis ATCC 6633 | 50-100 | luxuriant | Red to yellow |
| Salmonella Typhimurium ATCC 14028 | 50-100 | luxuriant | Red to yellow |
| Salmonella Abony NCTC 6017 | 50-100 | luxuriant | Red to yellow |
Storage and Shelf Life
Store below 30°C and the prepared medium at 2-8°C. Use before expiry date on the label.
Reference
- The United States Pharmacopoeia / National Formulary, 2012, The United States Pharmacopeial Convention Inc., Rockville, MD.
- Indian Pharmacopeia, 2010, Govt. of India, Ministry of Health and Family Welfare, New Delhi, India.
- Wright and Welch, 1959-60, Antibiotics Ann., 61.
| Product Name | Soyabean Casein Digest Medium w/ Mannitol, Sterile Powder |
|---|---|
| SKU | M1856G |
| Product Type | Regular |
| Physical Form | Sterile Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.The United States Pharmacopoeia / National Formulary, 2012, The United States Pharmacopeial Convention Inc., Rockville, MD. 2.Indian Pharmacopeia, 2010, Govt. of India, Ministry of Health and Family Welfare, New Delhi, India. 3.Wright and Welch, 1959-60, Antibiotics Ann., 61. |
| Customized Product Available | No |
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