M-FC Basal Medium

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SKU:
M1812
Recommended for enumeration of faecal coliform by membrane filtration technique with the addition of fluorogenic and chromogenic supplement.


M-FC Basal Medium is recommended for enumeration of faecal coliform by membrane filtration technique with the addition of fluorogenic and chromogenic supplement. The chromogenic substrate, 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide (BCIG) in the medium is cleaved by the presence of enzyme ß- glucuronidase in Escherichia coli thereby producing blue coloured colonies. The MUG Supplement in the medium is cleaved by the enzyme ß-glucuronidase of Escherichia coli to release 4- methylumbelliferone which produces visible blue–green fluorescence under long UV light. This medium can be used by addition of either of the two supplements or in combination for the confirmation of Escherichia coli.
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Product Name M-FC Basal Medium
SKU M1812
Product Type Regular
Physical Form Powder
Origin Animal
Packaging type HDPE
References 1.Collee J.G., Fraser A.G., Marmion B.P., Simmons A., (Eds) Mackie and McCartney, Practical Medical Microbiology 1996,14th Edition, Churchill Livingstone.2.Ciebin, Brodsky, Eddington, Horsnell, Choney, Palmateer, Ley, Joshi and Shears. 1995.Appl. Environ. Microbiol.3.Eaton A.D., Clesceri L.S. and Greenberg A.E. (ed.), 1995, Standard methods of examination of Water and Wastewater, 19thEdition., American Public Health Association, Washington,D.C.
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