Listeria Oxford Medium Base, Modified is recommended for isolation and differentiation of Listeria species from clinical and food specimens. Cycloheximide is used to reduce fungal contamination; cefotetan and fosfomycin are inhibitors of bacterial overgrowth. Acriflavin, colistin sulphate and lithium chloride inhibit bacteria other than Listeria species. Alternatively moxalactam (FD266) can be added which inhibits both gram-positive and gram-negative bacteria. Listeria monocytogenes hydrolyses esculin thereby blackening the medium.
1. Curtis G. D. W., Mitchell R. G, King A. F., Griffin E. J., 1989,Lett. Appl. Microbiol.,8:952.Van Netten P., Peroles I., Van de Mosdik A., Curtis G. D. W., Mossel D. A. A, 1988, Int. J. Food Microbiol., 6:187.3.Hayes P. S, Feeley J. L, Groves L. M, Ajello G. W. and Fleming D. W, 1986, Appl. Environ. Microbiol., 51:438.4.Fernandez G. J. F., Dominguez R. L., Vazzuez B. J. A., Rodriguez F.E. F., Briones D. V., Blanco L. J. L., Suarez F. G.,1986, Can. J. Microbiol., 32:149.