Modified Tergitol Agar Base w/ 1.0% Agar

Modified Tergitol Agar Base w/ 1.0% Agar is recommended for the detection and enumeration of coliform and heat-tolerant bacteria in water from different sources by membrane filter method. It is a selective and differential medium for the detection and enumeration of coliforms in water. Tergitol 7 acts as a selective agent which inhibits gram positive organisms and minimises swarming of Proteus species. Lactose fermentation is observed by change in colour of bromo thymol blue, the pH indicator.TTC is rapidly reduced by coliforms except Escherichia coli and Enterobacter aerogenes to insoluble formazan which gives red colour to the colonies. The lactose fermenters show greenish yellow colonies with yellow zones while lactose non-fermenters show red colonies surrounded by blue zones.