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Glucose Agar, Modified is the medium for maintenance of stock cultures of a variety of microorganisms.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Peptone special | 5.000 |
| Glucose | 10.000 |
| Agar | 20.000 |
Final pH (at 25°C): 6.5±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 35.0 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Distribute into tubes or flasks. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Pour into sterile Petri plates or distribute into tubes as desired.
Principle And Interpretation
Glucose Agar, Modified is a simple medium supporting growth of wide variety of organisms. It can be used for maintenance of stock cultures of microorganisms (1). It has been demonstrated as a universal medium used for germination of spores of fungus - Colletotrichum lindemuthianum (2). It has also been used to study growth profile of Histoplasma capsulatum and Candida albicans along with Sabouraud dextrose agar to study effect of pH and concentration of glucose (3).
It has glucose as immediate source of carbon. Special peptone provides necessary nitrogenous sources for growth of organisms. Agar acts as a solidifying agent.
Quality Control
Appearance: Cream to yellow coloured homogeneous free flowing powder
Gelling: Firm, comparable with 2.0% Agar gel
Colour and Clarity of prepared medium: Light amber coloured, clear to slightly opalescent gel forms in Petri plates / tubes
Reaction: Reaction of 3.5% w/v aqueous solution at 25°C. pH : 6.5±0.2
pH: 6.30-6.70
Cultural Response: M1679: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
Cultural Response
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli ATCC 25922 | 50-100 | good-luxuriant | >=70% |
| Enterobacter aerogenes ATCC 13048 | 50-100 | good-luxuriant | >=70% |
| Pseudomonas aeruginosa ATCC 25923 | 50-100 | good-luxuriant | >=70% |
| Candida albicans ATCC 10231 | 50-100 | luxuriant | >=70% |
| Histoplasma capsulatum ATCC 10230 | 50-100 | luxuriant | >=70% |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
Reference
- Atlas, R.M.1997. Handbook of microbiological media, 2nd edition, CRC Press Inc. USA.
- E. Drijfhout and J. Jansen 1989. European Journal of Plant Pathology. Vol.95, No 2. p.119
- Mathur, R.S., Barnett, H.L. & Lilly, V.G., 1950. Phytopathology Vol. 40: 104.
- Bartlett, G.W. etal., 1968, Journal of Bacteriology Vol 95.No.6 p. 2171.
| Product Name | Glucose Agar, Modified |
|---|---|
| SKU | M1679 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Atlas, R.M.199 |
| Customized Product Available | No |
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