Letheen Agar w/ Triton X-100 is recommended for screening cosmetic products for microbial contamination. It supports luxuriant growth of most organisms including detection of yeast and moulds. Triton X-100 acts as a surfactant. Lecithin and polysorbate 80 to the medium enables the recovery of bacteria from materials containing residues of disinfectant compounds or preservatives used in cosmetics.Cosmetics contain preservatives hence they should be at least partially inactivated during the plating and this medium helps in dilution as well as neutralizing. Enrichment in this medium should be done for 7 days at 30-32°C and then subcultured on Letheen Agar, Modified (M946) and/or MacConkey Agar (M081).
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