Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Casein Soya Agar, Modified is recommended as a general-purpose medium for cultivation of various microorganisms.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Pancreatic digest of casein | 14.500 |
| Papaic digest of soyabean meal | 5.000 |
| Sodium chloride | 5.000 |
| Growth factors | 1.500 |
| Agar | 14.000 |
Final pH ( at 25°C) 7.3±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 40 grams in 1000 ml distilled water. Mix thoroughly and heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. DO NOT OVERHEAT. Cool to 45-50°C. For preparation of blood plates, add 5-10% v/v sterile, defibrinated blood to the sterile medium. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Casein Soya Agar, Modified is a nutrient medium, which can be used as a base medium as well as an unsupplemented medium. Casein Soya Agar, Modified is a modified version of Tryptone Soya Agar, which is supplemented with 5-10% sterile blood. This medium is used for cultivation of fastidious organisms and for determining haemolytic reactions. The medium can be used in differentiation of Streptococcus species. The medium is supplemented with growth factors to achieve a better growth of fastidious microorganisms. Blood is the most common additive for Tryptone Soya Agar and it can be added at different concentrations between 5 and 15%.
Pancreatic digest of casein and papaic digest of soyabean meal in the medium provide organic nitrogen and amino acids. Sodium chloride maintains osmotic balance of the medium. Sheep blood stimulates excellent growth and aids in the formation of appropriate hemolytic reactions of fastidious organisms. The medium with 5% horse blood supplies both X and V factors that are growth requirements for certain organisms; e.g. Haemophilus influenzae. Haemolytic reactions displayed by defibrinated horse blood differ from those of sheep blood (1).
Quality Control
Appearance Cream to yellow homogeneous free flowing powder
Gelling Firm, comparable with 1.4% Agar gel
Colour and Clarity of prepared medium Basal medium: Yellow to tan coloured clear to slightly opalescent gel. After addition of 5-7%w/v sterile defibrinated blood : Cherry red coloured opaque gel forms in Petri plates
Reaction Reaction of 4.0% w/v aqueous solution at 25°C. pH : 7.3±0.2
pH 7.10-7.50
Cultural Response M1615: Cultural characteristics observed with added 5% w/v sterile defibrinated blood after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Haemolysis |
|---|---|---|---|---|
| Streptococcus pneumoniae ATCC 6305 | 50-100 | good-luxuriant | >=70% | alpha |
| Escherichia coli ATCC 25922 | 50-100 | good-luxuriant | >=70% | beta |
| Listeria monocytogenes ATCC 19115 | 50-100 | good-luxuriant | >=70% | beta(+/-) |
| Staphylococcus aureus ATCC 25923 | 50-100 | good-luxuriant | >=70% | beta |
| Staphylococcus aureus ATCC 6538p | 50-100 | good-luxuriant | >=70% | beta |
| Streptococcus pyogenes ATCC 19615 | 50-100 | good-luxuriant | >=70% | beta |
| Streptococcus pyogenes ATCC 49117 | 50-100 | good-luxuriant | >=70% | beta |
| Shigella dysenteriae ATCC 9361 | 50-100 | good-luxuriant | >=70% | none |
| Candida albicans ATCC 10231 | 50-100 | good-luxuriant | >=70% | none |
Storage and Shelf Life
Store below 30°C in tighly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
Reference
- Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology, 8th Ed., American Society for Microbiology, Washington, D.C. ,,
| Product Name | Casitose Soya Agar, Modified |
|---|---|
| SKU | M1615 |
| Customized Product Available | No |
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