M-PC Broth

Composition**

Final pH ( at 25°C) 7.0±0.2

**Formula adjusted, standardized to suit performance parameters

Directions

Suspend 17 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.

Principle And Interpretation

Bacteria can be removed from liquids by passing them through filters with very small pores that trap bacteria, but in general, not Mycoplasmas or viruses (1). The membrane filter technique is highly reproducible, can be used to test relatively large sample volumes, and usually yields numerical results more rapidly. This technique is extremely useful in monitoring drinking water and a variety of natural waters (2).

M-PC Broth used for enumerating microorganisms by membrane filtration has composition similar to Plate Count Agar (M091), except agar. Also M-PC Broth has the media ingredients in double concentrations, than the agar medium (1). This medium can therefore be used as a general purpose non-selective medium to determine total bacterial counts from food and water sample by the membrane filtration procedure.

Casein enzyme hydrolysate provides carbon and nitrogen source. Yeast extract serves as a source of trace elements, vitamin B complex nutrients and essential amino acids. Dextrose serves as the carbon source.

Quality Control

Appearance Cream to yellow homogeneous free flowing powder

Colour and Clarity of prepared medium Light yellow coloured clear solution without any precipitate.

Reaction Reaction of 1.7% w/v aqueous solution at 25°C. pH : 7.0±0.2

pH 6.80-7.20

Cultural Response M1610: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.

Storage and Shelf Life

Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.

Reference

1. Eaton A. D., Clesceri L. S. and Greenberg A. W., (Eds.), 2005, Standard Methods for the Examination of Water and Wastewater, 21st Ed., APHA, Washington, D.C.
2. Collins C. H., Lyne P. M., Grange J. M., 1995, Collins and Lynes Microbiological Methods, 7th Ed., Butterworth Heinemann.