Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Arabinose Agar is recommended for selective isolation of Enterococcus faecium from faeces, sewage and water samples.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Peptone, special | 23.000 |
| Corn starch | 1.000 |
| Sodium chloride | 5.000 |
| Arabinose | 10.000 |
| Phenol red | 0.100 |
| Agar | 15.000 |
Final pH ( at 25°C) 7.8±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 27.05 grams in 500 ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C and aseptically add the rehydrated contents of 1 vial of Enterococcus faecium Selective Supplement (FD226). Mix well and pour into sterile Petri plates.
Principle And Interpretation
Arabinose Agar Base is based on arabinose fermentation by Enterococcus species. Enterococcus species are becoming increasingly important agents of human diseases, largely because of their resistance to antimicrobial agents (1), thereby becoming a major cause of nosocomial infections (2). The use of selective medium for the isolation of Enterococci has been previously reviewed and Arabinose Agar Base with Enterococcus faecium Selective Supplement (FD226) (containing cephalexin and aztreonam) is recommended for the selective isolation of E. faecium from heavily contaminated sites (3).
Peptone special serves as a source of nitrogen, carbon and essential growth nutrients. Corn starch neutralizes the toxic metabolites formed where as sodium chloride maintains the osmotic equilibrium. Arabinose is the fermentable carbohydrate. E. faecium ferments arabinose, producing acidic conditions. This acidity developed is visualized as a colour change from red to yellow, due to the phenol red indicator in the medium.
Quality Control
Appearance Light yellow to pink homogeneous free flowing powder
Gelling Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium Red coloured clear to slightly opalescent gel forms in Petri plates.
Reaction Reaction of 5.41% w/v aqueous solution at 25°C. pH : 7.8±0.2
pH 7.60-8.00
Cultural Response
M1576: Cultural characteristics observed with added Enterococcus faecium Selective Supplement(FD226) after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of Colony |
|---|---|---|---|---|
| Escherichia coli ATCC 25922 | >=10³ | inhibited | 0% | |
| Enterococcus faecalis ATCC 29212 | 50-100 | good-luxuriant | >=50% | colourless-pink |
| Enterococcus faecium ATCC 19434 | 50-100 | good-luxuriant | >=50% | yellow |
| Enterococcus hirae ATCC 10541 | 50-100 | good-luxuriant | >=50% | colourless-pink |
| Pseudomonas aeruginosa ATCC 27853 | >=10³ | inhibited | 0% | |
| Staphylococcus aureus ATCC 25923 | >=10³ | inhibited | 0% |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on label.
Reference
- Eliopoulous G. M., Eliopoulous C. T., 1990, Eur. J. Clin Microbiol. Infect. Dis., 9:118-126
- Chenoweth C., Schaberg D., Eur. J. Clin. Microbiol. Infect. Dis., 1990, 9:80-89
- Ford M., Perry J. D. and Gould F. K., 1994, J. Clin. Microbiol., 32:2999-3001.
| Product Name | Arabinose Agar Base |
|---|---|
| SKU | M1576 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Eliopoulous G. M., Eliopoulous C. T., 1990, Eur. J. Clin Microbiol. Infect. Dis., 9:118-126 |
| Customized Product Available | No |
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