Pseudomonas Solanacearum Medium

Pseudomonas Solanacearum Medium is recommended for the cultivation of Pseudomonas solanacearum.

Composition

Formula adjusted, standardized to suit performance parameters.

Directions

Suspend 33 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Distribute into tubes or flasks. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates or leave in tubes.

Principle And Interpretation

Pseudomonas solanacearum is one of the worlds most potent phytopathogenic pseudomonad (1). It falls under non-fluorescent group of Pseudomonads. Pseudomonas solanacearum is commonly isolated from soil and is often an internal resident of plant tissues. Pseudomonas Solanacearum Medium is accepted as a cultivation and maintenance medium for P. solanacearum (2).Peptic digest of animal tissue and casein enzymic hydrolysate in the medium provide carbon and nitrogen sources required for the good growth of the bacterium. Glucose is the energy source.

Quality Control

Appearance: Cream to yellow homogeneous free flowing powder

Gelling: Firm, comparable with 1.7% Agar gel

Colour and Clarity of prepared medium: Light yellow coloured clear to slightly opalescent gel forms in Petri plates or tubes

Cultural Response

M1381: Cultural characteristics observed after an incubation at 25-30°C for 18-48 hours.

Storage and Shelf Life

Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.

Reference

1. Balows A., Truper H. G., Dworkin M. Harder W. and Schleifer K. H., (Eds.), 1992, The Prokaryotes, 2nd Ed., Vol. III : 3104, Springer-Verlag Publ. N.Y.
2. Atlas R. M., 1997, Handbook of Microbiological Media, 2nd Edition, Lawrence C. Parks (Ed.), CRC Press.