Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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SD Agar is used for the growth of yeasts for molecular biology purposes.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Ammonium sulphate | 5.000 |
| Dextrose | 20.000 |
| Biotin | 0.00002 |
| Calcium pantothenate | 0.002 |
| Folic acid | 0.000002 |
| Inositol | 0.010 |
| Niacin | 0.0004 |
| p-Amino benzoic acid (PABA) | 0.0002 |
| Pyridoxine hydrochloride | 0.0004 |
| Riboflavin | 0.0002 |
| Thiamine hydrochloride | 0.0004 |
| Boric acid | 0.0005 |
| Copper sulphate | 0.00004 |
| Potassium iodide | 0.0001 |
| Ferric chloride | 0.0002 |
| Manganese sulphate | 0.0004 |
| Sodium molybdate | 0.0002 |
| Zinc sulphate | 0.0004 |
| Potassium dihydrogen phosphate | 0.850 |
| Dipotassium hydrogen phosphate | 0.150 |
| Magnesium sulphate | 0.500 |
| Sodium chloride | 0.100 |
| Calcium chloride | 0.100 |
| Agar | 20.000 |
Final pH (at 25°C): 6.9±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 46.71 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
This medium is formulated as per Wickerham (2) for studying colonial and cellular morphology of yeasts. Dextrose provides on energy source, while the salts serve as a source of nutrition for metabolic reactions.
Quality Control
Appearance Cream to beige homogeneous free flowing powder
Gelling Firm, comparable with 2.0% agar gel.
Colour and Clarity Light amber coloured, opalescent gel forms in precipitate.
Reaction Reaction of 4.6% w/v aqueous solution at 25°C. pH 6.9 ± 0.2 pH: 6.9±0.2
pH 6.70-7.10
Cultural Response
M1371: Cultural characteristics observed after an incubation at 25-30°C for 48-72 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Cultural Response | |||
| Candida albicans ATCC 10231 | 50-100 | luxuriant | >=70% |
| Saccharomyces cerevisiae ATCC 9763 | 50-100 | luxuriant | >=70% |
Storage and Shelf Life
Store below 30°C in tightly closed container and prepared medium at 2-8 °C. Use before expiry date on label.
Reference
- Sherman, F., 1991, Meths. Enzymol. 194:3
- Wickerham, 1951, U.S. Dept. Agri. Tech. Bull. No. 1029.
| Product Name | SD Agar |
|---|---|
| SKU | M1371 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Chemically defined |
| Packaging type | HDPE |
| References | 1. Sherman, F., 1991, Meths. Enzymol. 194:3 |
| Customized Product Available | No |
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