M-Filter Rinse Broth

M-Filter Rinse Broth is used as a rinsing fluid in the membrane filtration procedure. This medium complies with the formulation as described in USP. After membrane filtration it is often necessary to rinse the membrane filter in order to remove residues of liquid sample materials. Use of this medium is recommended if the sample contains higher hydrocarbons or fats. The rinsing fluid protects the microorganisms retained by the filter from physiological shock, thus enabling microbes to grow rapidly. Polysorbate 80 emulsifies the carbohydrate and fats without harming the microorganisms. If the sample contains large quantities of carbohydrates or fats, additional quantities of polysorbate 80 up to 9.0 gm/litre can be added to the broth medium in accordance with USP recommendations. After filtering the liquid sample, rinse the filter 3 times with 100 ml portions of the M-Filter Rinse Broth, then complete the test in usual way.