L-Ornithine Decarboxylase Broth

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M1223I
Recommended for detection of the ability of microorganisms to decarboxylate ornithine. The composition and performance of this media are as per specifications laid down in in ISO /TS 22964: 2017.


L-Ornithine Decarboxylase Broth is recommended for detection of the ability of microorganisms to decarboxylate ornithine. The composition and performance of this media are as per specifications laid down in in ISO /TS 22964: 2017. This medium is based on the Taylors modification and is recommended by the ISO Committee and APHA for the detection of ornithine decarboxylation by Cronobacter sakazakii. If the organism produces the appropriate enzyme, i.e. decarboxylase, the amino acid (ornithine) in the medium is degraded, yielding a corresponding amine. Decarboxylation of ornithine yields putrescine. The production of this amine elevates the pH of the medium towards alkalinity, changing the color of the indicator from yellow to purple or violet. If the organism does not produce the appropriate enzyme, the medium remains acidic or yellow in colour.
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Product Name L-Ornithine Decarboxylase Broth
SKU M1223I
Product Type Regular
Physical Form Powder
Origin Animal Free (Microbial)
Packaging type HDPE
References 1. American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., Washington D.C.2.International Organization for Standardization. Microbiology of the food chain- Horizontal method for the detection ofCronobacter spp. Draft ISO/ TS 22964, 2017 (E).3.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition.4.Jorgensen, J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W.(2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.5.MacFaddin J.F., 2000, Biochemical Tests for Identification of Medical Bacteria, 3rd Ed., Lippincott, Williams and Wilkins,Baltimore.6.Moeller V., 1955, Acta Pathol. Microbiol. Scand. 36 (2): 158-1727.Salfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods, 5thEd., American Public Health Association, Washington, D.C.8.Smith D.T., Coant N.F., Willett H.P., Zinssers Microbiology, 14th Ed., New York: Appleton-Century-Crofts, 1968:118-1199.Taylor W.I., 1961, Appl. Microbiol., 9:487.
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