This medium is based on the formula described by FDA BAM for the detection of fluorescein production , a water soluble, chloroform insoluble fluorescent pigment by Pseudomonas species. The medium enhances the elaboration of fluorescein by Pseudomonas and inhibits the pyocyanin formation. The fluorescein pigment diffuses from the colonies of Pseudomonas into the agar and shows yellow fluorescent colouration.
1.FDA, U.S. 1998.Bacteriological Analytical Manual. 8 ed. Gaithersburg, MD: AOAC International. 2.MacFaddin, J. F. 198 5.Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria vol. 1. Baltimore: Williams and Wilkins.