Meat Extract w/ Peptone (Revised as Pepted M Broth)

Intended Use:

For cultivation and maintenance of Alcaligenes species.

Composition**

**Formula adjusted, standardized to suit performance parameters

# Equivalent to Meat extract

Directions

Suspend 28 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Distribute into tubes or flasks. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Principle And Interpretation

Alcaligenes are non-fermentative gram-negative bacilli. They exist in soil and water and have been isolated from many types of clinical specimens like urine, blood, spinal fluid, pleural fluid, wounds, abscesses and faeces (1). These are non saccharolytic microbes having simple nitrogenous growth requirements (2). Peptone with HM extract (Pepted M Broth) is recommended for the cultivation and maintenance of Alcaligenes species (3).

HM extract along with peptone provides necessary nitrogenous compounds and other nutrients for growth. Sodium chloride maintains osmotic equilibrium.

Type of specimen

Clinical samples - urine, blood, spinal fluid, pleural fluid, wounds, abscesses,faeces etc.; Dairy samples; Water samples

Specimen Collection and Handling:

For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5).

For dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (6).

For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(7)

After use, contaminated materials must be sterilized by autoclaving before discarding.

Warning and Precautions :

In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.

Limitations :

• Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
• Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
• Further serological and biochemical testing is required for complete identification.

Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

Quality Control

Appearance Cream to yellow homogeneous free flowing powder

Colour and Clarity of prepared medium Yellow coloured clear solution without any precipitate

Cultural Response

Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.

Storage and Shelf Life

Store between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.

Disposal

User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).

Reference

1. Finegold S. M. and Martin W. J., 1982, Bailey and Scotts Diagnostic Microbiology, 6th Ed., Pg. 258-59, The CV Mosby Company, London.
2. Koneman E. W., Allen S. D., Dowell V. R., Sommers H. M., 1983, Colour Atlas and Textbook of Diagnostic Microbiology, 2nd Ed., J. B. Lippincott, Company, Philadelphia, pg. 133.
3. Atlas R. M., 2004, Handbook of Microbiological Media, Lawrence C. Parks (Ed.), 3rd Edition, CRC Press.
4. Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
5. Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.
6. Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed., APHA Inc., Washington, D.C.
7. Lipps WC, Braun-Howland EB, Baxter TE, eds. Standard methods for the Examination of Water and Wastewater, 24th ed. Washington DC:APHA Press; 2023.