Urea Agar Base (Christensen) (Autoclavable)

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M112
For the detection of urease production, particularly by members of the genus Proteus.


This medium detects urease activity by all rapidly urease-positive Proteus organisms and also by other members of Enterobacteriaceae that exhibits a delayed urease reaction. Prolonged incubation may cause alkaline reaction in the medium. A medium without urea serves as negative control to rule out false positive results. Also, all urea test media rely on the alkalinity formation and so they are not specific for determining the absolute rate of urease activity. The utilization of proteins may raise the pH to alkalinity due to protein hydrolysis and excess of amino acids liberation results in false positive reaction.
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Product Name Urea Agar Base (Christensen) (Autoclavable)
SKU M112
Product Type Regular
Physical Form Powder
Origin Animal
Packaging type HDPE
References 1. Christensen W. B., 1946, J. Bacteriol., 52:461.
2.MacFaddin J. F., 2000, Biochemical Tests for Identification of Medical Bacteria, 3rd Ed., Williams and Wilkins, Baltimore.Md.
3.Farmer J. J. III, McWhorter A. C., Huntley G. A., Catignani J., J. Clin. Microbiol. 1975: 1 (1): 106-107.
4.MacFaddin J. F, 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williamsand Wilkins, Baltimore, Md.
5.American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., WashingtonD.C.
6.Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination ofFoods, 5th Ed., American Public Health Association, Washington, D.C.
7.Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water andWastewater, 23rd ed., APHA, Washington, D.C.
8.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition.
9.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1.
10.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc., Washington, D.C.
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