Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Wurtz Medium is used as a non-selective medium for isolation and differentiation of lactose-fermenting bacteria.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Meat peptone | 5.000 |
| Beef extract | 3.000 |
| Sodium chloride | 5.000 |
| Lactose | 10.000 |
| Bromo thymol blue | 0.075 |
| Agar | 15.000 |
Final pH (at 25°C): 7.0±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 38.08 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates.
Principle And Interpretation
The family Enterobacteriaceae consists of a large group of organisms all of which ferment glucose. Those, which ferment lactose, are grouped together as “coliform bacteria". Pathogenic serotypes of Escherichia coli present a particular problem and their isolation has always been a difficult process. Wurtz Medium is a non-selective medium employed for the growth, and the differentiation of Enterobacteriaceae from clinical samples, especially when suspected with the presence of pathogenic E. coli (1).
The medium contains meat peptone and beef extract, which provide essential growth nutrients. Lactose is the fermentable sugar and bromothymol blue acts as pH indicator. Lactose fermenters form yellow coloured colonies, while the lactose-non-fermenting Enterobacteriaceae grows as blue colonies due to the alkalization of the medium.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel.
Colour and Clarity of prepared medium: Green coloured clear to slightly opalescent gel forms in Petri plates.
Reaction: Reaction of 3.81% w/v aqueous solution at 25°C. pH: 7.0±0.2
pH: 6.80-7.20
Cultural Response
M1065: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of colony |
|---|---|---|---|---|
| Cultural Response | ||||
| Escherichia coli ATCC 25922 | 50-100 | luxuriant | >=70% | yellow |
| Klebsiella pneumoniae ATCC 13883 | 50-100 | luxuriant | >=70% | yellow |
| Proteus vulgaris ATCC 13315 | 50-100 | luxuriant | >=70% | blue |
| Salmonella Typhi ATCC 6539 | 50-100 | luxuriant | >=70% | blue |
| Salmonella Enteritidis ATCC 13076 | 50-100 | luxuriant | >=70% | blue |
| Shigella flexneri ATCC 12022 | 50-100 | luxuriant | >=70% | blue |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
Reference
- Corry J. E. L., Curtis G. D. W. and Baird R. M., (Eds.), Culture Media for Food Microbiology, Vol. 34, Progress in Industrial Microbiology, 1995, Elsevier, Amsterdam.
| Product Name | Wurtz Medium |
|---|---|
| SKU | M1065 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Corry J. E. L., Curtis G. D. W. and Baird R. M., (Eds.), Culture Media for Food Microbiology, Vol. 34, Progress in IndustrialMicrobiology, 1995, Elsevier, Amsterdam. |
| Customized Product Available | No |
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