Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Diluent TSB with Cap 4 can be used for the detection and enumeration of microorganisms for products of sanitary importance, water miscible cosmetics, water insoluble products fatty products containing antimicrobials or preservatives.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Pancreatic digest of casein | 17.000 |
| Papaic digest of soyabean meal | 3.000 |
| Sodium chloride | 5.000 |
| Dextrose | 2.500 |
| Dibasic potassium phosphate | 2.500 |
| Cap 4/100 ml | 25.000ml |
| Lecithin | 2.000 |
| Peptone | 1.000 |
| Tween 80 | 14.000 |
| Tamol | 3.000 |
| Distilled water | 75.000ml |
Formula adjusted, standardized to suit performance parameters
Directions
Each tube contains 9ml of TSB medium w/ 25% Cap 4. Add 1 ml of the sample to obtain a 10-fold dilution of the initial sample. Further serial dilutions can be carried out as desired.
Principle And Interpretation
Tryptone Soya Broth is recommended by various pharmacopeias as a sterility testing and as a microbial limit testing medium (1, 2, 3). This medium is a highly nutritious medium used for cultivation of a wide variety of organisms (4).
The combination of pancreatic digest of casein and papaic digest of soyabean meal makes the medium nutritious by providing amino acids and long chain peptides for the growth of microorganisms. Dextrose and dibasic potassium phosphate serve as the carbohydrate source and the buffer, respectively in the medium. Sodium chloride maintains the osmotic balance of the medium.
Cap 4 added to the medium is a mixture of dispersant and neutralizers that are reported to disperse the hydrophobic copolymer and to inactivate residual disinfectants in the sample(5).
This medium can thus be used for detection and enumeration of microbial counts in a wide variety of samples.
Quality Control
Appearance: Sterile clear TSB + Cap 4 medium in glass tubes.
Colour: Dark amber coloured, clear solution.
Quantity of Medium: 9 ml of medium in glass tubes.
pH: 7.10-7.50
Sterility test: Passes release criteria
Growth Promotion Test: Growth Promotion is carried out in accordance with the harmonized method of USP/EP/BP/JP.
Stability test
Light yellow coloured clear solution without any precipitation or sedimentation at room temperature for 7 days
Growth promoting properties
Clearly visible growth of microorganism comparable to that previously obtained with previously tested and approved lot of medium occurs at the specified temperature for not more than the shortest period of time specified inoculating <=100 cfu(at 30-35°C for 18-24 hours for bacteria and 5 days for fungal).
Sterility Testing + Validation
The medium is tested with suitable strains of microrganisms inoculating <=100cfu and incubating at 20-25°C for not more than 3 days in case of bacteria and not more than 5 days in case of fungi.
Cultural response
Cultural characteristics observed after an incubation at specified temperature and time.
Growth promoting
| Organism | Inoculum (CFU) | Growth | Incubation temperature | Incubation period |
|---|---|---|---|---|
| Escherichia coli ATCC 8739 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Escherichia coli ATCC25922 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Staphylococcus aureus ATCC 6538 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Staphylococcus aureus ATCC 25923 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Escherichia coli NCTC 9002 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Pseudomonas aeruginosa ATCC 9027 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Pseudomonas aeruginosa ATCC 27853 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Bacillus subtilis ATCC 6633 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Micrococcus luteus ATCC 9341 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Salmonella Typhimurium ATCC 14028 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Salmonella Abony NCTC 6017 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Streptococcus pneumoniae ATCC 6305 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
Sterility Testing - Growth promotion+Validation
| Organism | Inoculum (CFU) | Growth | Incubation temperature | Incubation period |
|---|---|---|---|---|
| Pseudomonas aeruginosa ATCC 27853 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Bacillus subtilis ATCC 6633 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Salmonella Typhimurium ATCC 14028 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Salmonella Abony NCTC 6017 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Candida albicans ATCC2091 | 50-100 | luxuriant | 30-35 °C | <=5 d |
| *Aspergillus brasiliensis ATCC 16404 | 50-100 | luxuriant | 30-35 °C | <=5 d |
| Staphylococcus aureus ATCC 6538 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Staphylococcus aureus ATCC 25923 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Escherichia ATCC 25922 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Escherichia coli NCTC 9002 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Pseudomonas aeruginosa ATCC 9027 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Micrococcus luteus ATCC 9341 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Streptococcus pneumoniae ATCC 6305 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Candida albicans ATCC 10231 | 50-100 | luxuriant | 30-35 °C | <=5 d |
| Escherichia coli ATCC 8739 | 50-100 | luxuriant | 20-25 °C | <=3 d |
Storage and Shelf Life
Store between 2-8°C. Use before expiry date on the label.
References
- MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams & Wilkins, Baltimore, M.d.
- The United States Pharmacopeia, 2008, USP31/NF26, The United States Pharmacopeial Convention, Rockville, MD.
- Indian Pharmacopeia, 2007, Govt. of India, Ministry of Health and Family Welfare, New Delhi, India.
- Forbes B. A., Sahm D. F. and Weissfeld A. S., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc. St. Louis, Mo.
- Brummer, 1976, Appl. Environ. Microbiol., 32:80.
| Product Name | Diluent TSB with Cap4 |
|---|---|
| SKU | LQ506 |
| Customized Product Available | No |
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