Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended use
Recommended for the detection of aerobic and anaerobic fermentation of glucose.
Composition**
| Ingredients | g/L |
|---|---|
| Peptone | 2.000 |
| Sodium chloride | 5.000 |
| Dipotassium hydrogen phosphate | 0.300 |
| Glucose (Dextrose) | 10.000 |
| Bromothymol blue | 0.030 |
| Agar | 3.000 |
| Final pH (at 25°C) | 7.1±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Label the ready to use LQ296X bottle. Inoculate the sample and incubate at specified temperature and time. In an addition for anaerobic condition, a set of tubes with 5mm paraffin oil may be layered on surface of medium for the differentiation of oxidative and fermentative organisms.
Principle And Interpretation
Hugh Leifson Medium was formulated by Hugh and Leifson (1). They described the taxonomic significance of fermentative and oxidative metabolism of carbohydrates in gram-negative intestinal bacteria. It is recommended by BIS (2) for the isolation and cultivation of Vibrio cholerae and other Vibrio species which cause food poisoning. The medium contains a high concentration of carbohydrate and low concentration of peptone to avoid the possibility of an aerobic organism utilizing peptone and producing an alkaline condition which would neutralize slight acidity produced by an oxidative organism (3). Dipotassium phosphate promotes fermentation and acts as pH controlling buffer. Agar concentration enables the determination of motility and aids in distribution of acid throughout the tube produced at the surface of medium. Oxidative organisms produce acid in unsealed tube with little or no growth and no acid formation in sealed tube while fermentative organisms produce acid in both sealed and unsealed tubes.
Type of specimen
Isolated microorganism
Specimen Collection and Handling:
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic Use. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Other biochemical tests must be performed in conjunction for confirmation.
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Hugh Leifson Medium in glass botttle
Colour
Green Coloured solution
Quantity of Medium
10 ml of medium in glass bottle
pH
6.90-7.00
Sterility Check
Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Aerobic fermentation | Anaerobic fermentatoin |
|---|---|---|
| Vibrio cholerae ATCC 15748 | acid (yellow) and gas production, positive reaction | acid (yellow) and gas production, positive reaction |
| Vibrio parahaemolyticus ATCC 17802 (00037*) | greenish blue, negative reaction | acid (yellow) and gas production, positive reaction |
Key- (*) Corresponding WDCM number
Storage and Shelf Life
On receipt store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | Hugh Leifson Medium |
|---|---|
| SKU | LQ296X |
| Customized Product Available | No |
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