Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use:
Recommended for the preservation and maintainance of bacterial cultures.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Tryptone | 17.000 |
| Soya peptone | 3.000 |
| Sodium chloride | 5.000 |
| Dextrose (Glucose) | 2.500 |
| Dipotassium hydrogen phosphate | 2.500 |
| Glycerol | 150.0 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Label the ready to use LQ278II bottle. Inoculate the bacterial culture and maintain for long term preservation at cold temperatures..
Principle And Interpretation
Tryptone Soya Broth is a highly nutritious medium used for cultivation of a wide variety of organisms (1). The combination of Tryptone and soya peptone makes the medium nutritious by providing nitrogenous, carbonaceous substances, amino acids and long chain peptides for the growth of microorganisms. Dextrose/glucose serve as the carbohydrate source and dibasic potassium phosphate buffer the medium. Sodium chloride maintains the osmotic balance of the medium. Glycerol plays a major role as an anti freezing agent. Hence it is recommended for maintenance of cultures as glycerol serves as an cryopreservant.
Type of specimen
Pure Isolates
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Biochemical characterization is necessary to be performed on colonies from pure cultures for further identification.
- This medium may not support the maintenance of fastidious organisms and fungal strains.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile Tryptone Soya Broth w/15% Glycerol in a glass vial.
Colour
Light yellow coloured clear solution
Quantity of Medium
2 ml of medium in glass vial.
Sterility test
Passes release criteria
pH
7.10-7.50
Cultural response
Cultural characteristics observed after an incubation at 30-35°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant |
| Salmonella Abony NCTC 6017 (00029*) | 50-100 | luxuriant |
| Pseudomonas aeruginosa ATCC 9027 (00026*) | 50-100 | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | 50-100 | luxuriant |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant |
| Escherichia coli ATCC 8739 (00012*) | 50-100 | luxuriant |
| Bacillus subtilis subsp. spizizenii ATCC 6633 (00003*) | 50-100 | luxuriant |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant |
Key :* -Corresponding WDCM numbers
Storage and Shelf Life
On receipt store between 15-25°C. Use before expiry date on the label Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | Tryptone Soya Broth w/ 15% glycerol |
|---|---|
| SKU | LQ278II |
| Customized Product Available | No |
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