Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for propagation of pathogenic cocci and other fastidious organisms associated with blood culture work and allied pathological investigations.
Composition**
| Ingredients | g/L |
|---|---|
| HM infusion powder # | 12.500 |
| BHI powder | 5.000 |
| Proteose peptone | 10.000 |
| Dextrose (Glucose) | 2.000 |
| Sodium chloride | 5.000 |
| Disodium hydrogen phosphate | 2.500 |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Calf brain infusion from
Directions
Label the ready to use LQ210C bottle. Inoculate the sample and Incubate at specified temperature and time.
Principle And Interpretation
BHI Medium is useful for cultivating a wide variety of microorganisms since it is a highly nutritive medium. It is also used to prepare the inocula for antimicrobial susceptibility testing. BHI Broth is a modification of the original formulation of Rosenow, where he added pieces of brain tissues to dextrose broth (1). BHI Broth is also the preferred medium for anaerobic bacteria, yeasts and moulds (2,3). This medium is nutritious and well buffered to support the growth of wide variety of organisms (3,4,5).
Proteose peptone, HM infusion powder and BHI powder serve as sources of carbon, nitrogen, essential growth factors, amino acids and vitamins. Dextrose serves as a source of energy. Disodium phosphate helps in maintaining the buffering action of the medium whereas sodium chloride maintains the osmotic equilibrium of the medium.
Type of specimen
Clinical samples : pathological samples; Food and Dairy samples
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (4,6,7). For clinical samples follow appropriate techniques for handling specimens as per established guidelines (8,9). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns .
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile clear BHI broth in glass bottle.
Colour: Light to medium amber coloured medium
Quantity of medium: 100 ml of medium in glass bottle
Sterility Check: Passes release criteria
pH: 7.20-7.60
Cultural Response
Cultural characteristics observed after incubation at 35 - 37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Neisseria meningitidis ATCC 13090 | 50-100 | Luxuriant |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | Luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | Luxuriant |
| Candida albicans ATCC 10231 (00054*) | 50-100 | Luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | Luxuriant |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | Luxuriant |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (8,9).
| Product Name | BHI Broth |
|---|---|
| SKU | LQ210C |
| Customized Product Available | No |
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