Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
For sterility testing of highly viscous or gelatinous substances such as salves, ointments and other cosmetic product.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Casein enzymic hydrolysate | 20.000 |
| Azolectin | 5.000 |
**Formula adjusted, standardized to suit performance parameters
Directions
Label the ready to use LQ203D bottle. Inoculate the sample and Incubate at specified temperature and time.
Principle And Interpretation
T.A.T. Broth is prepared according to the formula recommended by United States Food and Drug Administration (1) for enrichment and further isolation and cultivation of gram-negative bacteria in cosmetics, tropical drugs and in the sterility testing of viscous or gelatinous substances. It is especially adapted for the testing of cosmetics.
Cosmetics and pharmaceutical products are subject to contamination during manufacturing and subsequent use by consumers (2). Preservatives are used in aqueous products to make them self-sterilizing for vegetative bacteria, yeasts and moulds, and bacteriostatic or bactericidal for spores (2).
Casein enzymic hydrolysate provides the nitrogen, vitamins, amino acids and carbon in T.A.T. Broth Base. Azolectin and polysorbate 20 neutralize preservatives in the cosmetics or pharmaceutical products, allowing bacteria to grow.
Prepare decimal dilutions of the sample to be tested from 10-1 to 10-6. Inoculate 1 gram (1 ml) sample and 1 ml of each dilution into 40 ml of T.A.T. Broth (3). After incubation, subculture the growth on MacConkey Agar (M081) and TSI Agar (M021).
Quality Control
Appearance
Sterile T.A.T. Medium in a glass bottle.
Colour
Light yellow coloured clear to slightly opalescent solution
Quantity of Medium
500 ml of medium in glass bottle.
pH
7.00- 7.40
Sterility test
Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) |
Growth |
|---|---|---|
| Bacillus subtilis ATCC 6633 | 50-100 | good-luxuriant |
| Candida albicans ATCC 10231 | 50-100 | good-luxuriant |
| Pseudomonas aeruginosa ATCC 27853 | 50-100 | fair-good |
| Salmonella Typhi ATCC 6539 | 50-100 | good-luxuriant |
| Staphylococcus aureus ATCC 25923 | 50-100 | good-luxuriant |
| Staphylococcus aureus ATCC 6538 | 50-100 | good-luxuriant |
| Pseudomonas aeruginosa ATCC 9027 | 50-100 | fair-good |
Storage and Shelf Life
Store between 2-8°C. Use before expiry date on the label.
| Product Name | T.A.T. Broth |
|---|---|
| SKU | LQ203D |
| Customized Product Available | No |
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