Phosphate Buffer, pH 7.2

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Availability: In stock
Only %1 left
SKU:
LQ165C


Intended use

Used as a diluent as recommended by FDA BAM and USP.

Composition

Ingredients g/L
Potassium dihydrogen phosphate 34.000

The pH is adjusted to 7.2 with 1N NaOH and the volume made to 1000 ml with distilled water.

Final Phosphate Buffer, pH 7.2: 1ml of the above solution is added to 800 ml of purified water and sterilized.

Directions

Label the ready to use LQ165C bottle. Inoculate 50-100 cfu sample and Incubate at specified temperature and time.

Principle And Interpretation

Phosphate buffer pH 7.2 is formulated as described in FDA BAM & USP (1,2). The phosphate buffer is required for the antibiotic preparation used in antibiotic assay. It is used in preparation of dilutions.

Type of specimen

Food samples, Pharmaceutical samples

Specimen Collection and Handling

For food samples, follow appropriate techniques for sample collection and processing as per guidelines (1).

For pharmaceutical samples, follow appropriate techniques for sample collection and processing as per guidelines (2). After use, contaminated materials must be sterilized by autoclaving before discarding.

Warning and Precautions

Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.

Limitations

  • It is incompatible with calcium ions.

Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

Quality Control

Appearance
Sterile clear Phosphate Buffer Solution pH 7.2 in glass bottles

Colour
Colourless clear solution.

Quantity
100 ml of solution in glass bottles

pH
7.20

Sterility Check
Passes release criteria

Storage and Shelf Life

On receipt store between 15-30°C. Use before expiry date on the label. Product performance is best if used stated expiry within period.

Disposal

User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).

More Information
Product Name Phosphate Buffer, pH 7.2
SKU LQ165C
Customized Product Available No
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