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Phosphate Buffer, pH 7.2
Intended use
Used as a diluent as recommended by FDA BAM and USP.
Composition
| Ingredients | g/L |
|---|---|
| Potassium dihydrogen phosphate | 34.000 |
The pH is adjusted to 7.2 with 1N NaOH and the volume made to 1000 ml with distilled water.
Final Phosphate Buffer, pH 7.2: 1ml of the above solution is added to 800 ml of purified water and sterilized.
Directions
Label the ready to use LQ165C bottle. Inoculate 50-100 cfu sample and Incubate at specified temperature and time.
Principle And Interpretation
Phosphate buffer pH 7.2 is formulated as described in FDA BAM & USP (1,2). The phosphate buffer is required for the antibiotic preparation used in antibiotic assay. It is used in preparation of dilutions.
Type of specimen
Food samples, Pharmaceutical samples
Specimen Collection and Handling
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (1).
For pharmaceutical samples, follow appropriate techniques for sample collection and processing as per guidelines (2). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- It is incompatible with calcium ions.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile clear Phosphate Buffer Solution pH 7.2 in glass bottles
Colour
Colourless clear solution.
Quantity
100 ml of solution in glass bottles
pH
7.20
Sterility Check
Passes release criteria
Storage and Shelf Life
On receipt store between 15-30°C. Use before expiry date on the label. Product performance is best if used stated expiry within period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Phosphate Buffer, pH 7.2 |
|---|---|
| SKU | LQ165C |
| Customized Product Available | No |

