Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Intended Use
Recommended for cultivation of wide variety of microorganisms with the addition of carbohydrates it can be used for fermentation studies.
Composition
| Ingredients | g/L |
|---|---|
| Tryptone | 17.000 |
| Soya peptone | 3.000 |
| Sodium chloride | 5.000 |
| Dipotassium hydrogen phosphate | 2.500 |
| Dextrose (Glucose) | 2.500 |
| Bromocresol purple | 0.010 |
| Final pH (at 25°C) | 7.3±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Label the ready to use LQ148XX bottle. Inoculate 50-100 cfu sample and Incubate at specified temperature and time.
Principle And Interpretation
Soyabean Casein Digest Medium is a nutritious medium that will support the growth of a wide variety of microorganisms, including common aerobic, facultative and anaerobic bacteria and fungi (1-4). It can also be used as a general, all purpose cultivation medium (3). When tested for the growth of organisms in presence of indicator like bromocresol purple, the colour of the medium changes from purple to yellow. With the addition of carbohydrates it can be also used for the fermentation studies of fastidious and non-fastidious organisms.
Tryptone and soya peptone provides necessary amino acids and other complex nitrogenous substances. Dextrose serves as an energy source. Sodium chloride maintains the osmotic equilibrium. Dipotassium phosphate acts as a buffer to control pH.
Type of specimen
Environmental monitoring- Sterility testing in manufacturing process.
Specimen Collection and Handling
For environmental monitoring, follow appropriate techniques for sample processing in case of viscous materials as mentioned under sterility (1,4).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- 1. Further biochemical testing is required on colonies of pure culture for complete identification.
- 2.Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile clear Soyabean Casein Digest Medium w/BCP in glass bottle.
Colour: Purple coloured clear solution
Quantity of Medium: 20 ml of medium in glass bottle.
pH: 7.10-7.50
Sterility Check: Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours (Candida albicans incubated at 20-25°C for 2-7 days).
| Organism | Inoculum (CFU) | Growth | Colour of medium |
|---|---|---|---|
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant | yellow |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | good-luxuriant | yellow |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | good-luxuriant | yellow |
| Bacillus spizizenii ATCC 6633 (00003*) | 50-100 | good-luxuriant | yellow |
Key: (*) Corresponding WDCM numbers.
**Formerly known as Bacillus subtilis subsp. spizizenii
Storage and Shelf Life
On receipt store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Soyabean Casein Digest Medium w/BCP |
|---|---|
| SKU | LQ148XX |
| Customized Product Available | No |
Write Your Own Review