Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
For the cultivation and maintenance of recombinant strains of Escherichia coli and may be used for routine cultivation of not particularly fastidious microorganisms.
Composition
| Ingredients | Gms/Litre |
|---|---|
| Casein enzymic hydrolysate | 10.000 |
| Yeast extract | 5.000 |
| Sodium chloride | 10.000 |
Formula adjusted, standardized to suit performance parameters
Directions
Label the ready to use LQ118 bottle. Inoculate the sample and Incubate at specified temperature and time.
Principle And Interpretation
Luria Bertani Broth, Miller (1) is slightly different with double amount of sodium chloride as compared to original media described by Lennox (2) for cultivation and maintenance of recombinant strains of Escherichia coli The media is nutritionally rich for the growth of pure cultures of recombinant strains. Strains derived from Escherichia coli K12 are deficient in Vitamin B synthesis which are further modified by specific mutation to create auxotrophic strains and are therefore unable to grow on nutritionally deficient media. Casein enzymic hydrolysate provides peptides and peptones while Vitamin B complex is provided by yeast extract. Sodium chloride provides sodium ions for membrane transport and also maintains the osmotic equilibrium of the medium.
Quality Control
Appearance: Sterile clear Luria Bertani broth in bottles.
Colour: Yellow to amber coloured solution.
Quantity of medium: 10ml of medium in bottles.
Reaction: 7.30-7.70
Sterility test: Passes release criteria
Cultural Response
Cultural characteristics observed after incubation at 35 - 37°C for 18-24 hours.
| Organism | Growth | Inoculum (CFU) |
|---|---|---|
| Escherichia coli ATCC 23724 | luxuriant | 50-100 |
| Escherichia coli ATCC 25922 | luxuriant | 50-100 |
| Escherichia coli DH5 alpha MTCC 1652 | luxuriant | 50-100 |
Storage and Shelf Life
Store between 2-8°C. Use before expiry date on the label.
| Product Name | Luria Bertani Broth |
|---|---|
| SKU | LQ118 |
| Customized Product Available | No |
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