Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use:
Recommended for determining the efficiency of sanitization of containers, equipments, surfaces, water miscible cosmetics.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Tryptone | 17.000 |
| Soya peptone | 3.000 |
| Sodium chloride | 5.000 |
| Dextrose (Glucose) | 2.500 |
| Dipotassium hydrogen phosphate | 2.500 |
| Lecithin | 0.700 |
| Polysorbate 80 (Tween 80) | 5.000 |
| Histidine | 0.500 |
| Sodium thiosulphate | 0.500 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Label the ready to use LQ117 bottle. Inoculate 50-100 cfu sample and Incubate at specified temperature and time.
Principle And Interpretation
Soyabean Casein Digest Medium w/ LTHTh is used for the detection and enumeration of microorganisms for products of sanitary importance, water miscible cosmetics, products containing antimicrobials or preservatives (1). Soya peptone and Tryptone provide nitrogenous compounds and other nutrients essential for microbial replication. Lecithin, polysorbate 80 (Tween 80) and thiosulphate act as neutralizing agents, they are reported to neutralize the activity of antimicrobial agents, quaternary ammonium compounds and parahydroxy benzoates. Sodium thiosulphate neutralizes mercurial, halogens, aldehydes etc. Histidine acts as a reducing agent. Collection of samples from areas before and after the treatment with disinfectant evaluates cleaning procedures in environmental sanitation. The presence and number of microorganisms is determined by the appearance of colonies on the agar surface (2).
Type of specimen
Swabs of containers, Equipment surfaces, Sanitary products, water miscible cosmetics.
Specimen Collection and Handling
For sanitary products, water miscible cosmetics follow appropriate techniques for handling specimens as per established guidelines (1, 4).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual strain of a microorganism may have unique growth requirements with respect to nutrients and physical conditions. Based on which the growth pattern of each varies on a medium and some even may display significant delay.
- Further biochemical and serological tests must be carried out for complete identification.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile Soyabean Casein Digest Broth w/LTHTh in bottles.
Colour: Light to medium Amber coloured clear solution
Quantity of medium: 10 ml of medium in bottles.
Sterility test: Passes release criteria
pH: 7.10-7.50
Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Growth | Growth w/ disinfectant |
|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | luxuriant | fair-good, (depends on concentration of quarternary ammonium compounds) |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | luxuriant | fair-good, (depends on concentration of quarternary ammonium compounds) |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | luxuriant | fair-good, (depends on concentration of quarternary ammonium compounds) |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 15-25°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | Soyabean Casein Digest Medium w/ LTHTh |
|---|---|
| SKU | LQ117 |
| Customized Product Available | No |
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