Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for the recovery of anaerobic and facultative microorganisms. Sterile, in glass bottles.
Composition
| Ingredients | g/L |
|---|---|
| Tryptone | 15.000 |
| Yeast extract | 5.000 |
| Dextrose (Glucose) | 5.500 |
| Sodium chloride | 2.500 |
| L-Cystine | 0.500 |
| Sodium thioglycollate | 0.500 |
| Final pH (at 25°C) | 7.1±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Label the ready to use blood culture bottle. Remove the Aluminium foil cap. Disinfect the part of the rubber stopper which is now exposed. Draw patient's blood with the sterile or disposable needle and syringe as explained in specimen collection and disposable column. Transfer the blood sample immediately into the culture bottle by puncturing the rubber stopper with the needle and injecting the blood. Venting: Use sterile venting needle (LA038). Keep the bottle in an upright position preferably in a biological safety cabinet, place an alcohol swab over the rubber stopper and insert the venting needle with filter through it. Insertion and withdrawal of the needle should be done in a straight line. discard the needle and mix the contents by gently inverting the bottle 2-3 times. Do Not vent the bottle for anaerobic cultures. Incubate at 35-37°C for 24-72 hours and further for seven days.
Principle And Interpretation
Alternative Thioglycollate Medium is formulated as described in the N.I.H. memorandum (1). It is recommended for the recovery of anaerobic and facultative microorganisms. Tryptone serves as a source of nitrogen and carbon compounds, long chain amino acids and other essential nutrients. Yeast extract serve as source of essential nutrients to the contaminants, if present. Dextrose serves as the energy source. Sodium chloride maintains the osmotic equilibrium of the medium whereas L-cystine, an amino acid, also serves as source of essential growth factors. Sodium thioglycollate and L-cystine lower the oxidation-reduction potential of the medium by removing oxygen to maintain a low Eh. Sodium thioglycollate also helps to neutralize the toxic effects of mercurial preservatives (2,3).
Type of specimen
Clinical: Blood
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- 1. Proper anaerobic conditions must be maintained for optimal recovery of organisms
- 2. Not recommended for paediatric use.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile clear Thioglycollate Broth in glass bottle
Colour
Light yellow coloured clear solution.
Quantity of Medium
70ml of medium in glass bottle (For Adult Use)
pH
6.90-7.30
Sterility Check
Passes release criteria
Cultural response
Cultural characteristics was observed after incubation at 35-37°C for 24-72 hours
| Organism | Inoculum (CFU) |
Growth under anaerobic conditions |
Growth under aerobic conditions |
|---|---|---|---|
| Clostridium sporogenes ATCC 19404 (00008*) | 50-100 | luxuriant | - |
| Clostridium sporogenes ATCC 11437 | 50-100 | luxuriant | - |
| Clostridium perfringens ATCC 13124 (00007*) | 50-100 | luxuriant | - |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | - | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | 50-100 | - | luxuriant |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | - | luxuriant |
| ^Pseudomonas paraeruginosa ATCC 9027 (00026*) | 50-100 | - | luxuriant |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | - | luxuriant |
| Escherichia coli ATCC 8739 (00012*) | 50-100 | - | luxuriant |
| Salmonella Abony NCTC 6017 (00029*) | 50-100 | - | luxuriant |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | - | luxuriant |
| Bacteroides fragilis ATCC 23745 | 50-100 | luxuriant | - |
| # Phocaeicola vulgatus ATCC 8482 | 50-100 | luxuriant | - |
Key: *Corresponding WDCM numbers.
^ Formerly known as Pseudomonas aeruginosa (#) Formerly known as Bacteroides vulgatus
Storage and Shelf Life
Store between 2-8°C. Use before expiry date on the label. Product performance is best ifused within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | Thioglycollate Broth |
|---|---|
| SKU | LQ007A |
| Customized Product Available | No |
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