Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended use
Surface Sampling Kit for Microbiological control in slaughter houses and meat processing plants.
Kit contents
- a) 1 Sterile swab individually packed.
- b)1 bottle with 40ml sterile diluent (M1062) Dey-Engley Neutralizing Broth)
- c)1 Sterile disposable square template 20cm² (5x4cm) in a zip lock bag.
Directions
- Select the areas to be sampled by placing the sterile disposable square template
- Dampen the sterile swab by using sterile diluent (M1062 - Dey-Engley Neutralizing Buffer).
- In case of moist surface, the swab can be used directly.
- Place the template against the area to be sampled, press it firmly and roll the swab in all directions, so that the entire area to be swabbed is covered.
- After the area has been swabbed, break off the wooden shaft of the swab and place the head of the swab in the diluent (Dey-Engley Neutralizing Buffer).
- For further processing of the sample the bag is transported to the laboratory under refrigerated conditions within 4 hours.
Principle And Interpretation
Sampling kits are used for microbiological control in slaughter houses and meat processing plants. This kits are also useful for environmental sampling of working areas and equipment control in general. Application areas: Food, Animal feed, Dairy industries, Meat processing plants, Large catering areas etc.
Type of specimen
Environmental samples
Specimen Collection and Handling
For environmental samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (Refer directions). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance:
- 1 Sterile swab individually packed.
- 1 bottle with 40ml sterile diluent (M1062) Dey-Engley Neutralizing Broth)
- 1 Sterile disposable square template 20cm² (5x4cm) in a zip lock bag.
Colour and Clarity
Purple to reddish purple coloured, opalescent solution (may have particulate precipitate) of Dey-Engley Neutralizing Broth (M1062)
Sterility Check
No growth is observed after 14 days for Bacteria at 30-35°C and for fungi at 20-25°C.
Cultural Response:
Cultural characteristics observed after an incubation at 35-37°C for 40-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Bacillus subtilis subsp. spizizenii ATCC 6633 (00003*) | 50-100 | luxuriant |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | luxuriant |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant |
| Escherichia coli ATCC 8739 (00012*) | 50-100 | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | 50-100 | luxuriant |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 2-8°C. Product performance is best when used within expiry period.
Disposal
Unseal the bag and add three chlorine tablets in each bag to decontaminate. Mix well and allow to stand for 30 minutes and then pour contents into sink drain, toilet or hole in the ground. Safely dispose the bag.
| Product Name | Surface-Swab-Kit (Wet & Dry Swab Method) |
|---|---|
| SKU | K083 |
| Customized Product Available | No |
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