HiPer® Antibody Capture ELISA Teaching Kit can be used for the determination of antibody concentration bound to immobilized antigen followed by binding of the antigen to the labeled secondary antibody and its detection by using appropriate substrate.
HiPer® Antibody Capture ELISA Teaching Kit can be used for the determination of antibody concentration bound to immobilized antigen followed by binding of the antigen to the labeled secondary antibody and its detection by using appropriate substrate. In this method, the test solution containing the unknown amount of antibody is added to the antigen bound wells. The unbound antibody is washed off. The antibody bound to the immobilized antigen is detected using a labelled secondary antibody. I is a type of competitive elisa.