HiPer® Southern Blotting Teaching Kit demonstrates the technique of Southern blotting and hybridization. iIt s used to identify and quantitate specific DNA sequences involving transfer of DNA onto nylon membrane
HiPer® Southern Blotting Teaching Kit is a commonly used method for the identification of DNA
fragments that are complementary to a known DNA sequence. It is a widely used technique in molecular biology for transfer of DNA molecules from an electrophoresis gel to a nitrocellulose or nylon membrane, and is carried out prior to detection of specific molecules by hybridization probing. In this method a DNA mixture is separated by agarose gel electrophoresis according to their size followed by transfer of the DNA bands to nitrocellulose/nylon membrane. Finally, the DNA of interest is probed for a specific sequence.